Abstract: In this study, the applicability of the Chinese national standard methods to detect vitamin B1, vitamin B2, vitamin B6, and niacin in infant formula for special medical purposes was explored. High performance liquid chromatography (HPLC) was used to determine vitamin B1, vitamin B2, vitamin B6 and niacin in eight samples of three types of infant formula foods for special medical-purpose and infant formula milk powder quality control samples in seven laboratories. The recovery rate was also measured. The results showed that the national standard methods were suitable for the determination of vitamin B1, vitamin B2, vitamin B6, and niacin in infant formula milk powder, and for the determination of vitamin B1, vitamin B2 and vitamin B6 in infant formula for special medical purposes. The recovery rate of vitamin B1 was low. The recovery rate of vitamin B2 in deep hydrolysis formula and amino acid formula products was low, and the test data for niacin was generally unsatisfactory. This study provides technical support for the establishment of methods for testing infant formula for special medical purposes.

Key words: high performance liquid chromatography; special medical purpose; infant formula food; vitamin B1; vitamin B2; vitamin B6; niachin