Multiple Real-time Polymerase Chain Reaction for Identification of Animal-derived Ingredients in Milk Products from Minor Dairy Species

doi:10.7506/spkx1002-6630-20200407-080

Abstract

Abstract: The aim of this study was to establish a multiple real-time polymerase chain reaction (real-time PCR) method for the rapid identification of animal-derived ingredients in milk products from minor dairy species. A multiple real-time PCR system was developed for eight dairy animal-derived ingredients, which allowed for the simultaneous detection of four target genes. The absolute sensitivity was 0.1–5 pg/μL and the limit of detection (LOD) was 0.1%. Besides, an efficient DNA extraction method for dairy products was established, and DNA extraction from 96 samples could be completed within 12 min after sample lysis, greatly shortening the sample pretreatment time. By using this method, we surveyed commercially available dairy products from minor dairy species, revealing that 36.36% of the samples were inaccurately labelled.

Key words: multiple real-time polymerase chain reaction; milk of non-bovine mammals; dairy animals; adulteration; identification

CLC Number:

TS252.7

YANG Yange, LI Li, WANG Dandan, LI Weixi, WANG Hongyue, LIU Mingchang, WU Yajun. Multiple Real-time Polymerase Chain Reaction for Identification of Animal-derived Ingredients in Milk Products from Minor Dairy Species[J]. FOOD SCIENCE, 2021, 42(16): 312-321.

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Multiple Real-time Polymerase Chain Reaction for Identification of Animal-derived Ingredients in Milk Products from Minor Dairy Species

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