Antioxidant and immunological activities of Sacha Inchi soluble dietary fiber

Abstract

Abstract: Sacha Inchi soluble dietary fiber (SISDF) was evaluated for its antioxidant and immunological activity. The structure of SISDF was identified by ion chromatography, gel chromatography and Fourier transform infrared spectroscopy. The results showed that the relative molecular weight of SISDF was 4.01×105 Da, which is a large polysaccharide with pyranose as the main chain. Among the monosaccharide composition, galacturonic acid had the largest content (46.49%), followed by rhamnose (18.95%), xylose (17.60%) and galactose (11.25%), etc. When the mass concentration of SISDF was 2 mg/mL, the scavenging rate of DPPH radicals was 68.57%, hydroxyl radicals was 69.83% and ABTS+ radicals was 70.35%. In the mass concentration range of 62.5~1000 μg/mL, SISDF enhanced the proliferative and phagocytic activities of RAW264.7 macrophages (P＜0.01) and increased the secretion levels of NO, IL-1β, IL-6 and TNF-α factors in RAW264.7 macrophages. Sacha Inchi soluble dietary fiber has good antioxidant activity and immune activity, and has the potential to be developed as a natural food antioxidant and anti-inflammatory nutraceutical.

Key words: Sacha Inchi, Dietary fiber, Antioxidant, Immune activity

ZHAO-Wenjun. Antioxidant and immunological activities of Sacha Inchi soluble dietary fiber[J]. FOOD SCIENCE, 0, (): 0-0.

References

[1]张启月, 张士凯, 郗良卿, 等.不同提取方法对樱桃酒渣水溶性膳食纤维结构、理化与功能性质的影响[J].食品科学, 2021, 42(7):98-105 [2]HUANG Jiayi, LIAO Jinsong, Qi Junru, et al.Structural and physicochemical properties of pectin-rich dietary fiber prepared from citrus peel[J].[J]., 2021, 110: 106140., 110(1):106140-106140[J].Food Hydrocolloids, 2021, 110(1):106-140 [3]DU Xiaojing, WANG Li, HUANG xin, et al.Effects of different extraction methods on structure and properties of soluble dietary fiber from defatted coconut flour[J].LWT-Food Science & Technology, 2021, 143(1):111031-111031 [4]薛山, 肖夏, 谢建山.超声波辅助酶法提取琯溪柚皮海绵层水溶性抗氧化膳食纤维工艺优化[J].食品工业科技, 2021, 42(01):197-203 [5]刁春仁.藕渣可溶性膳食纤维的提取分离、结构表征及其对肠道微生物的影响[D]. 江苏大学, 2019: 1-7. [6]林锦铭, 蔡尤林, 李俊健, 等.美藤果壳多酚物质的提纯及其抗氧化性[J].食品研究与开发, 2021, 42(5):106-112 [7]夏辉, 吕名蕊, 刘建, 等.油藤籽仁营养成分分析[J].粮食与食品工业, 2013, 20(02):9-11 [8]LI Shanshan, YANG Guang, YAN Jingmin, et al.Polysaccharide structure and immunological relationships of RG-I pectin from the bee pollen of Nelumbo nucifera[J].International Journal of Biological Macromolecules, 2018, 111(1):660-666 [9]王筱霏.黄芪多糖及其硫酸化修饰产物体内外抗炎活性研究[D]. 西北农林科技大学, 2014:4-24. [10]ZHANG Chenxiao, DAI Ziru.Immunomodulatory activities on macrophage of a polysaccharide from Sipunculus nudus L[J].Food and Chemical Toxicology, 2011, 49(11):2961-2967 [11]MA Mengmei, MU Taihua.Effects of extraction methods and particle size distribution on the structural,physicochemical,and functional properties of dietary fiber from deoiled cumin[J].Food Chemistry, 2016, 194(1):237-246 [12]TIAN Wenni, XIAO Nan, YANG Yunyun, et al.Structure,antioxidant and immunomodulatory activity of a polysaccharide extracted from Sacha Inchi seeds[J].International Journal of Biological Macromolecules, 2020, 162(1):116-126 [13]SONG Ya, ZHU Minqian, HAO Huili, et al.Structure characterization of a novel polysaccharide from Chinese wild fruits (Passiflora foetida) and its immune-enhancing activity[J].International Journal of Biological Macromolecules, 2019, 136(1):324-331 [14]尹震花, 张娟娟, 陈林, 等.补骨脂多糖的分离纯化、结构鉴定及其对巨噬细胞活力的影响[J].食品科学, 2019, 40(24):27-32 [15]杨林松, 钟慧, 王璐瑶, 等.怀牛膝多糖对细胞吞噬活性、表型和细胞因子表达的调节[J].常州大学学报自然科学版, 2020, 32(04):55-62 [16]王琴琴, 韩珊, 李新星, 等.毛蕊花苷对脂多糖诱导细胞炎症模型的作用及其机制研究[J].中草药, 2020, 51(16):4217-4222 [17]HUANG Shengquan, DING Shaodong, FAN Liuping.Antioxidant activities of five polysaccharides from Inonotus obliquus[J].International Journal of Biological Macromolecules, 2012, 50(5):1183-1187 [18]KEREH V G, UNTU I M, NAJOAN M, et al.Extraction of uronic acid from and its feeding effects on the immunity of Lohman chicken eggs[J].IOP Conference Series: Earth and Environmental Science, 2021, 788(1):0-0 [19]AI Zhiyi, YOU Ying, LI Wancong, et al.Enhanced uronic acid content,antioxidant,and anti-inflammatory activities of polysaccharides from ginseng fermented by Saccharomyces cerevisiae GIW‐1[J].Journal of Food Processing and Preservation, 2020, 44(11):012047-012047 [20]CHENG Li, ZHANG Xianmei, HONG Yan, et al.Characterisation of physicochemical and functional properties of soluble dietary fiber from potato pulp obtained by enzyme-assisted extraction[J].International Journal of Biological Macromolecules, 2017, 101(1):1004-1011 [21]LIU Chun, LIN Xiaolu, WAN Zhili, et al.The physicochemical properties,in vitro binding capacities and in vivo hypocholesterolemic activity of soluble dietary fiber extracted from soy hulls[J].Food & Function, 2016, 7(12):4830-4840 [22]WANG Sunan, FAN Zhu, YUKIO Kakuda.Sacha Inchi (Plukenetia volubilis L): Nutritional composition,biological activity,and uses[J].J].Food Chemistry, 2018, 265(1):316-328 [23]MONICA Benincasa, CRISTINA Lagatolla, LUCILLA Dolzani, et al.Biofilms from Klebsiella pneumoniae: Matrix Polysaccharide Structure and Interactions with Antimicrobial Peptides,[J].Microorganisms, 2016, 4(3):0-0 [24]谢三都, 陈惠卿, 周春兰, 等.橄榄渣膳食纤维理化和体外吸附特性及结构表征[J].食品与机械, 2019, 35(10):29-34 [25]马光路, 吕建波, 曹青.玉米秸秆中木质素、半纤维素和纤维素的组分分离研究[J].中国农业科技导报, 2015, 17(06):70-79 [26]GUIMOND Scott E, RUDD Timothy R, SKIDMORE Mark A, et al.Cations modulate polysaccharide structure to determine FGF-FGFR signaling: a comparison of signaling and inhibitory polysaccharide interactions with FGF-1 in solution[J].Biochemistry, 2009, 48(22):4772-4779 [27]CHANG S C, HSU B Y, CHEN B H.Structural characterization of polysaccharides from Zizyphus jujuba and evaluation of antioxidant activity[J].International Journal of Biological Macromolecules, 2010, 47(4):445-453 [28]ZHA Zhengqi, WANG Suyan, CHU Weihua, et al.Isolation,purification,structural characterization and immunostimulatory activity of water-soluble polysaccharides from Lepidium meyenii[J].Phytochemistry, 2018, 147(1):184-193 [29]黄雅婷.薄盖灵芝多糖的分离纯化、结构鉴定及抗肿瘤活性研究[D]. 广东药科大学, 2016:30-68. [30]GUO Lei, DAI Hongwei, MA Jiayu, et al.Isolation,structure characteristics and antioxidant activity of two water-soluble polysaccharides from Lenzites betulina[J].BMC chemistry, 2021, 15(1）,15（1):0-0 [31]SADEQ Hassan Al-Sherajia, AMIN Ismail, MOHD Yazid Manap, et al.Purification,characterization and antioxidant activity of polysaccharides extracted from the fibrous pulp of Mangifera pajang fruits[J].LWT - Food Science and Technology, 2012, 48(2):291-296 [32]ROSENAU Thomas, POTTHAST Antje, HELL Johannes.Cellulose Science and Technology: Chemistry, Analysis, and Applications[M]. John Wiley & Sons, Inc, 2018-11-29. DOI:10.1002/9781119217619. [33]HUA Mei, SUN Yinshi, SHAO Zijun, et al.Functional soluble dietary fiber from ginseng residue: Polysaccharide characterization,structure,antioxidant,and enzyme inhibitory activity[J].Journal of Food Biochemistry, 2020, 44(12):e13524-e13524 [34]YUAN Huamao, ZHANG Weiwei, LI Xuegang, et al.Preparation and in vitro antioxidant activity of kappa-carrageenan oligosaccharides and their oversulfated,acetylated,and phosphorylated derivatives[J].Carbohydrate Research, 2005, 340(4):685-692 [35]CHEN Ruizhan, LIU Zhiqiang, ZHAO Jimin, et al.Antioxidant and immunobiological activity of water-soluble polysaccharide fractions purified from Acanthopanax senticosu[J].Food Chemistry, 2011, 127(2):434-440 [36]LI Hui, WANG Xin, XIONG Qian, et al.Sulfated modification,characterization,and potential bioactivities of polysaccharide from the fruiting bodies of Russula virescens[J].International Journal of Biological Macromolecules, 2020, 154(1):1438-1447 [37]饶川艳, 聂梦琳, 莫明规, 等.广西巴马长寿人群饮食中种膳食纤维成分及抗氧化活性分析[J].食品安全质量检测学报, 2020, 11(19):6990-6999 [38]王琴琴, 韩珊, 李新星, 等.毛蕊花苷对脂多糖诱导细胞炎症模型的作用及其机制研究[J].中草药, 2020, 51(16):4217-4222 [39]张晓红, 董莉, 杨雅欣, 等.紫金龙乙醇组分对脂多糖诱导的细胞分泌炎症因子的影响[J].中国实验方剂学杂志, 2014, 20(21):149-152 [40]李潭, 孙一涵, 李国峰.根皮素对脂多糖诱导的细胞的体外抗炎作用机制[J].中国免疫学杂志, 2021, 37(07):812-818