A Fluorescent Sensing System Based on Genefinder and Aptamer for the Detection of Ochratoxin A

doi:10.7506/spkx1002-6630-20201016-144

Abstract

Abstract: A fluorescence sensing system based on the nucleic acid dye Genefinder and aptamer was constructed for the detection of ochratoxin A (OTA) in this study. A double-stranded structure was formed by binding the OTA aptamer to its complementary strand. Binding of Genefinder to double-stranded DNA (dsDNA) generated a strong fluorescent signal. In the presence of OTA, the aptamer bound to it to form a quadruplex structure, melting the double strands and leading to a decrease of the fluorescence intensity of Genefinder. The results showed that the linear range of the sensing system was 50 pg/L–300 ng/L, and the detection limit was 50 pg/L. The sensing system had a high selectivity for OTA and could be widely applied as a simple and fast method.

Key words: aptamer; ochratoxin A; Genefinder; fluorescence sensing system

CLC Number:

TS207.3

GUO Ting, YE Zhijie, ZHOU Hongyuan, ZHANG Yuhao, MA Liang. A Fluorescent Sensing System Based on Genefinder and Aptamer for the Detection of Ochratoxin A[J]. FOOD SCIENCE, 2021, 42(20): 305-309.

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A Fluorescent Sensing System Based on Genefinder and Aptamer for the Detection of Ochratoxin A

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