Abstract: An immunochromatographic assay (FMICA) using fluorescent microsphere-labeled antibody as the signal probe was developed for rapid quantitative detection of nitrofuran metabolite residues in aquatic products. The limits of detection (LOD) for NPAHD, NPAMOZ, NPAOZ, and NPSEM were 0.004, 0.01, 0.008 and 0.009 μg/L, and the linear ranges were 0.005–5, 0.01–10, 0.01–10, and 0.01–10 μg/L, respectively. The detection process took 10 min. The assay had good specificity. The spiked samples were detected by FMICA after derivatization treatment, and the recoveries of the four analytes ranged from 75.88% to 104.81%, with variation coefficients of less than 15%. Therefore, FMICA proved to be simple, fast, and low-cost and could be suitable for rapid quantitative detection of nitrofuran metabolites in aquatic products.

Key words: nitrofuran; fluorescent microsphere; quantitative detection; immunochromatographic assay; aquatic products