Analysis of volatile components of different varieties of Osmanthus fragrans extracts based on GC-MS and electronic nose technology combined with chemometrics methods

Abstract

Abstract: Gas chromatography-mass spectrometry (GC-MS) and electronic nose technology combined with chemometrics methods were used to analyze the volatile components and overall odor of different varieties of Osmanthus fragrans extracts. The results showed that there were 65 kinds and 8 kinds of volatile components in four kinds of Osmanthus fragrans extracts detected by GC-MS, including 28 kinds of alcohols, 6 kinds of ketones, 4 kinds of aldehydes, 4 kinds of phenols, 4 kinds of acids, 5 kinds of alkanes, 8 kinds of esters and 6 kinds of other substances. Among them, alcohols, ketones and esters were the main contribution substances of volatile components in Osmanthus fragrans extracts. The GC-MS Venn diagram and cluster heat diagram objectively showed that the types and contents of volatile components in the four kinds of Osmanthus fragrans extracts were significantly different. By partial least squares discriminant analysis (PLS-DA), nine distinctive volatile components of four Osmanthus fragrans extracts were identified, which were tetracontane, propanoic acid, 1-cyclohexene-1-propanol, 1-octacosanol, palmitic acid, 9,12-tetradecadien-1-ol, cyclohexanol, 2-Butanone, and stigmasterol. The results of electronic nose showed that W2W, W2S, W1W, W1S, W5S were the main sensors to distinguish the odor of different Osmanthus fragrans extracts. The overall odor differentiation of the four kinds of Osmanthus fragrans extracts was better, the volatile components of Liuyang golden osmanthus and Liuyang silver osmanthus extracts were more similar, and the Xianning golden osmanthus and Liuyang Orange osmanthus extracts were significantly different, which was consistent with the GC-MS cluster analysis results. The results showed that it was feasible to distinguish different varieties of Osmanthus fragrans extracts based on volatile substances.

Key words: Osmanthus fragrans extract , Volatile component , Electronic nose , Cas chromatography mass spectrometry , Chemometrics

CLC Number:

TS222+.1

References

[1] Tang W Z, Zhao Y Q. Research progress on chemical constituents of plants from Osmanthus L. and their pharmacology[J]. Chinese Traditional and Herbal Drugs, 2014, 45(4): 590-602. DOI: 10.7501/j.issn.0253-2670.
[2] Xin H, Wu B, Zhang H, et al. Characterization of volatile compounds in flowers from four groups of sweet osmanthus (Osmanthus fragrans) cultivars[J]. Canadian Journal of Plant Science, 2013, 93(5): 923-931. DOI: 10.1139/CJPS2012-333.
[3] Wang L M, Li M T, Jin W W, et al. Variations in the components of Osmanthus fragrans Lour. essential oil at different stages of flowering[J]. Food Chemistry, 2009, 114(1): 233-236. DOI: 10.1016/j.foodchem.2008.09.044.
[4] Liao X, Hu F, Chen Z. Identification and quantitation of the bioactive components in Osmanthus fragrans fruits by HPLC-ESI-MS/MS[J]. Journal of agricultural and food chemistry, 2018, 66(1): 359-367. DOI: 10.1021/acs.jafc.7b05560.
[5] Bingfeng, Hu, Xiali, et al. Chemical Composition Comparison of the Essential Oil from Four Groups of Osmanthus fragrans Lour. Flowers[J]. Journal of Essential Oil Bearing Plants, 2012. 15(5): 832-838. DOI: 10.1080/0972060X.2012.10644128.
[6] Li F, Huang Q. Analysis of fragrance composition in three cultivars of Osmanthus fragrans Albus group flower by gas chromatography-mass spectrometry[J]. Wuhan University Journal of Natural Sciences, 2011, 16(4): 342-348. DOI:10.1007/s11859-011-0761-8.
[7] Tang W, Wang B, Wang M, et al. Ultrasound-assisted extraction of Osmanthus fragrans fruit oil and evaluation of its fatty acid composition, physicochemical properties and antioxidant activity[J]. Journal of Applied Research on Medicinal and Aromatic Plants, 2021, 25: 100331. DOI: 10.1016/j.jarmap.2021.100331.
[8] 国内-国家标准-国家市场监督管理总局 CN-GB 食品安全国家标准食品添加剂桂花浸膏: GB 1886.24-2015: 1[S].
[9] 田怀香, 李凤华, 吴艳. 利用电子鼻分析不同品种的桂花香气[J]. 林业科技开发, 2014, 28(04): 93-96. DOI: 10.13360/j.issn.1000-8101.2014.04.024.
[10] 吴雷, 卢斌斌, 席辉, 孙世豪, 许建营, 柴国璧, 张启东, 宗永立. 桂花浸膏香气成分的GC-MS分析[J]. 河南工业大学学报(自然科学版), 2019, 40(03): 78-85. DOI: 10.16433/j.cnki.issn1673-2383.2019.03.014.
[11] 张璟琳, 李娟, 黄明泉, 孙宝国, 张玉玉. 3种桂花浸膏的挥发性物质分析[J]. 食品安全质量检测学报, 2016, 7(04): 1554-1560. DOI: 10.19812/j.cnki.jfsq11-5956/ts.2016.04.039.
[12] 彭旭怡, 郑经绍, 刘宇航, 许琳浣, 匡维阳, 黄苇. 基于电子鼻、顶空气相色谱-离子迁移谱分析比较不同杀菌处理紫米甜酒酿中的挥发性成分[J]. 现代食品科技, 2021, 37(07): 259-268. DOI: 10.13982/j.mfst.1673-9078.2021.7.1091.
[13] Khorramifar A, Rasekh M, Karami H, et al. Application of MOS Gas Sensors Coupled with Chemometrics Methods to Predict the Amount of Sugar and Carbohydrates in Potatoes[J]. Molecules, 2022, 27(11): 3508. DOI: 10.3390/molecules27113508.
[14] Chen J, Tao L, Zhang T, et al. Effect of four types of thermal processing methods on the aroma profiles of acidity regulator-treated tilapia muscles using E-nose, HS-SPME-GC-MS, and HS-GC-IMS[J]. Lwt, 2021, 147: 111585. DOI: 10.1016/j.lwt.2021.111585.
[15] 王永瑞, 柏霜, 罗瑞明, 王松磊. 基于电子鼻、GC-MS结合化学计量学方法鉴别烤羊肉掺假[J]. 食品科学, 2022,43(04):291-298. DOI: 10.7506/spkx1002-6630-20210307-089.
[16] MELUCCI, DORA, BENDINI, ALESSANDRA, TESINI, FEDERICA, et al. Rapid direct analysis to discriminate geographic origin of extra virgin olive oils by flash gas chromatography electronic nose and chemometrics[J]. Food Chemistry, 2016, 204(Aug.1): 263-273. DOI: 10.1016/j.foodchem.2016.02.131.
[17] Yan T, Lin J, Zhu J, et al. Aroma analysis of Fuyun 6 and Jinguanyin black tea in the Fu'an area based on E-nose andGC–MS[J]. European Food Research and Technology, 2022, 248(4): 947-961. DOI: 10.1007/s00217-021-03930-8.
[18] Song J, Han J, Fu L, et al. Assessment of characteristics aroma of heat pump drying (HPD) jujube based on HS-SPME/GC–MS and e-nose[J]. Journal of Food Composition and Analysis, 2022, 110: 104-402. DOI: 10.1016/j.jfca.2022.104402.
[19] 郭永跃, 马君义, 吕孝飞, 闫辉强, 郭俊炜, 邓煜. 基于电子鼻技术鉴别陇南初榨橄榄油[J]. 中国粮油学报, 2021, 36(08): 94-100. DOI: 10.3969/j.issn.1003-0174.2021.08.016.
[20] 王俊斐, 张露平, 张运民, 刘耀武. 基于电子鼻技术鉴别不同产地半夏药材[J]. 现代中药研究与实践, 2021, 35(01): 1-3+8. DOI: 10.13728/j.1673-6427.2021.01.001.
[21] 马琦, 伯继芳, 冯莉, 佴逸凡, 王小晶, 李梅, 徐怀德. GC-MS结合电子鼻分析干燥方式对杏鲍菇挥发性风味成分的影响[J].食品科学, 2019, 40(14): 276-282. DOI: 10.7506/spkx1002-6630-20180904-046.
[22] 陆宽, 王雪雅, 孙小静, 霍昕, 高玉琼, 冯发进, 杜成兴. 电子鼻结合顶空SPME-GC-MS联用技术分析贵州不同品种辣椒发酵后挥发性成分[J]. 食品科学, 2018, 39(04): 199-205. DOI: 10.7506/spkx1002-6630-201804030.
[23] Usuki T, Munakata K. Extraction of Essential Oils from the Flowers of Osmanthus fragrans var. aurantiacus Using an Ionic Liquid[J]. Bulletin of the Chemical Society of Japan, 2017, 90(10): 1105-1110. DOI: 10.1246/bcsj.20170202.
[24] Bingfeng, Hu, Xiali, et al. Chemical Composition Comparison of the Essential Oil from Four Groups of Osmanthus fragrans Lour. Flowers[J]. Journal of Essential Oil Bearing Plants, 2012. 15(5): 832-838. DOI: 10.1080/0972060X.2012.10644128.
[25] Lei G, Mao P, He M, et al. Water-soluble essential oil components of fresh flowers of Osmanthus fragrans Lour[J]. Journal of EssEntial oil rEsEarch, 2016, 28(3): 177-184. DOI: 10.1080/10412905.2015.1114532.
[26] Bwa B, Fei L, Yba B, et al. Traditional uses, phytochemical constituents and pharmacological properties of Osmanthus fragrans: A review[J]. Journal of Ethnopharmacology, 2022, 293: 115-273. DOI: 10.1016/j.jep.2022.115273.
[27] Burdock G A. Fenaroli's handbook of flavor ingredients[M]. CRC press, 2016. DOI: 10.1201/9781439847503.
[28] 钱蕾, 杨程, 侯亚龙. 基于主成分分析和聚类分析讨论不同桂花浸膏的香气品质[J]. 香料香精化妆品, 2021(05): 20-27. DOI: 10.3969/j.issn.1000-4475.2021.05.004.
[29] 李欢康, 杨佳玮, 刘文玉, 魏长庆. 不同工艺核桃油挥发性物质比对及关键香气成分表征[J]. 食品科学, 2021, 42(16): 185-192. DOI: 10.7506/spkx1002-6630-20200901-006.
[30] Fu J, Hou D, Wang Y, et al. Identification of floral aromatic volatile compounds in 29 cultivars from four groups of Osmanthus fragrans by gas chromatography–mass spectrometry[J]. Horticulture, Environment, and Biotechnology, 2019, 60(4): 611-623. DOI: 10.1007/s13580-019-00153-5.
[31] 吴雷, 席辉, 赵龙, 孙世豪, 宗永立, 李河霖, 张逸寒, 马涛涛, 柴国璧, 张启东. 桂花浸膏中长链酯的测定及对香气成分释放的影响[J]. 烟草科技, 2020, 53(07): 62-69. DOI: 10.16135/j.issn1002-0861.2019.0205.
[32] 黄河, 黄婷, 王媚, 赵金松. 白酒中邻苯二甲酸酯塑化剂问题研究进展[J]. 酿酒科技, 2022(01): 97-103. DOI: 10.13746/j.njkj.2021148.
[33] Deng C, Song G, Hu Y. Application of HS‐SPME and GC‐MS to Characterization of Volatile Compounds Emitted from Osmanthus Flowers[J]. Annali di Chimica, 2004, 94(12). 921-927. DOI: 10.1002/adic.200490114.
[34] 徐春晖, 王远兴. 基于GC-MS结合化学计量学方法鉴别3种江西名茶[J]. 食品科学, 2020, 41(20): 141-150. DOI: 10.7506/spkx1002-6630-20190925-309.
[35] 尹洪旭, 杨艳芹, 姚月凤, 等. 基于气相色谱-质谱技术与多元统计分析对不同栗香特征绿茶判别分析[J]. 食品科学, 2019, 40(4): 192-198. DOI: 10.7506/spkx1002-6630-20180120-276.