Differences in Enzymatic Properties between Mannanases from Aspergillus fumigatus (AfMan5A) and Rhizopus microsporus (RmMan134C) and Their Synergy in the Degradation of Polysaccharides

doi:10.7506/spkx1002-6630-20220105-030

Abstract

Abstract: This study aimed to analyze the differences in the enzymatic properties of mannanases from Aspergillus fumigatus HBHF5 (AfMan5A, belonging to the GH5 family) and Rhizopus microsporus GZHF10 (RmMan134C, belonging to the GH134 family), and to explore their potential to synergistically degrade polysaccharides when used in combination. The results showed that the enzymatic properties of AfMan5A and RmMan134C were quite different. The optimum temperature and pH of AfMan5A were 60 ℃ and 6.0, respectively, while those of RmMan134C were 35 ℃ and 5.0, respectively. Cu2+, Zn2+, Mn2+ showed inhibitory activity against both enzymes, while Mg2+, Fe3+ and Li+ could promote the activity of AfMan5A, but slightly inhibit the activity of RmMan134C. RmMan134C exhibited a broader spectrum of substrates than AfMan5A, and the best substrate for both of them was konjac glucomannan. Furthermore, they did not synergistically act on mannan-based substrates but showed a good synergistic effect in the degradation of microcrystalline cellulose, with a synergy coefficient of 6.1. This study analyzed the difference in properties of GH5 and GH134 family mannanases and the synergistic effect of degrading polysaccharides, and provided a certain theoretical support for the further application and promotion of mannanases.

Key words: GH5 family; GH134 family; β-mannanase; enzymatic properties; synergy

CLC Number:

Q814

LIU Xue, LU Haiqiang, WANG Yuyin, TIAN Hongtao, GU Xinxi. Differences in Enzymatic Properties between Mannanases from Aspergillus fumigatus (AfMan5A) and Rhizopus microsporus (RmMan134C) and Their Synergy in the Degradation of Polysaccharides[J]. FOOD SCIENCE, 2022, 43(24): 145-153.

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Differences in Enzymatic Properties between Mannanases from Aspergillus fumigatus (AfMan5A) and Rhizopus microsporus (RmMan134C) and Their Synergy in the Degradation of Polysaccharides

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