Cloning and expression of a novel GH134 β-mannanase gene from thermophilic fungus Rhizopus microsporus and its application in juice processing

Abstract

Abstract: A thermophilic fungus HBFH10 was screened from Luzhou-flavor Daqu and identified as Rhizopus microsporus. A new β-mannanase gene (RmMan134) from a thermophilic fungus of Rhizopus microsporus HBHF10, was cloned, expressed and characterized. The cloned RmMan134 gene had a total length of 552 bp and no intron, encoding 183 amino acids and a stop codon with an estimated molecular mass of approximately 18.5 kDa. Herein, the RmMan134 was investigated and found to be modified by N-glycosylation. The deduced amino acid sequence of RmMan134 revealed the existence of a 19-residue signal peptide module and a glycoside hydrolase family 134 (GH134) catalytic module. The β-mannanase RmMan134 showed the most active at pH 6.0, temperature of 50?C, respectively. And it had high thermal stability and pH stability. When konjac gum was used as substrate, The Km and Vmax values of RmMan134 were 0.66 mg/mL and 57.1 μmol/(min·mg), respectively. After various fruit juices were treated with RmMan134, the clarity of the orange juice increased by 23.8%, followed by apple juice (11%) and peach juice (7%). Moreover, the RmMan134 significantly increased the yield of grape juice by about 7%. Overall, the RmMan134 might have potential applications in the juice and fruit processing.

Key words: Daqu, Thermophilic fungi, Rhizopus microsporus, Mannanase, GH134 family, Juice?processing

Wei Chen. Cloning and expression of a novel GH134 β-mannanase gene from thermophilic fungus Rhizopus microsporus and its application in juice processing[J]. FOOD SCIENCE, 0, (): 0-0.

References

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