Effect and Mechanism of Andrias Davidianus Peptide Supplementation Combined with Treadmill Exercise on Weight Loss in Obese Rats

Abstract

Abstract: Purpose: To evaluate the effect and underlying mechanism of Andrias davidianus peptide (ADP) supplementation combined with treadmill exercise on obesity. Methods: In this study, obesity model rats were fed with high-fat diet, and then randomly divided into model group (HFD), treadmill exercise group (Ex), Andrias davidianus peptide group (ADP) and Andrias davidianus peptide combined with treadmill exercise group (ADP+Ex). The rats in the Ex group were given a 6-week treadmill exercise training. The rats in the ADP group were administrated with Andrias davidianus peptide (ADP) by gavage for 6 weeks. The rats in the ADP+Ex group were performed ADP supplementation combined with treadmill exercise training. The normal SD rats were used as the control (CN) group. The body weight and food intake were documented each week, the level of circulating GLP-1, NPY, Irisin, Leptin, triglycerides (TG), total cholesterol (TC), low density lipoprotein (LDL) and high-density lipoprotein (HDL) were investigate by ELISA analysis. Western blots were used to detect the expression of appetite regulated protein (NPY and POMC) and energy metabolism related protein. Results: ADP supplementation combination with treadmill exercise resulted in a more significant reduction in body weight, fat accumulation around inguinal and epididymal and food intake, along with the content of circulating TG, TC and LDL induced by high-fat diet (HFD). In addition, ELISA results revealed that combination of ADP supplementation and treadmill exercise reduced the level of circulating NPY and leptin, conversely, enhanced GLP-1 and Irisin concentration in serum of obese rats. Likewise, western blots analysis also showed that ADP supplementation combined treadmill exercise largely elevated the expression of appetite inhibition related protein (POMC) and energy metabolism related proteins, while down-regulated the appetite-enhancing protein expression (NPY). Conclusion: Collectively, our research provides a new promising strategy to alleviate HFD-induced obesity.

Key words: Andrias davidianus peptide, exercise, appetite, energy metabolism

CLC Number:

TS201.4

Jin QIAN Shaohui Jia. Effect and Mechanism of Andrias Davidianus Peptide Supplementation Combined with Treadmill Exercise on Weight Loss in Obese Rats[J]. FOOD SCIENCE, 0, (): 0-0.

References

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