Metabolic Engineering of Escherichia coli for Fermentative Production of β-nicotinamide Mononucleotide

Abstract

Abstract: In order to realize efficient the fermentative production of β-Nicotinamide mononucleotide (β-NMN) by Escherichia coli, the modular metabolic engineering strategies were designed. Firstly, eight enzymes involved in nicotinamide (NAM) and β-NMN pathway metabolism were inactivated to reduce the additional consumption of precursors and products by chassis cells. Secondly, by introducing BcNaiP, BmPnuC, Prs and Nampt, deleting regulatory protein PurR, the engineered strain N12' can accumulate 0.34 g/L β-NMN in shake-flask fermentation. Then, by comparison and screening, we found that the Nampt enzyme from Comamonadaceae bacteriumChitinphaga Pinensis had higher activity and less burden on the chassis cells. By further strengthening the expression level of BmPnuC and Prs, the β-NMN titer of the engineered strain N18 increased to 1.36 g/L in shake-flask fermentation. Finally, after fed-batch fermentation for 38 h, β-NMN titer reached 10.2 g/L, and the molar conversion rate of NAM to β-NMN was 74.5%. The β-NMN fermentation strain constructed in this study has the advantages of clear genetic background, no nutritional defects, no induction, and has a good industrial prospect.

Key words: β-Nicotinamide mononucleotide, Escherichia coli, Nicotinamide phosphoribosyl transferase, Metabolic modification, Fermentation

CLC Number:

Q939.97

XU Qingyang. Metabolic Engineering of Escherichia coli for Fermentative Production of β-nicotinamide Mononucleotide[J]. FOOD SCIENCE, 0, (): 0-0.

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