Simultaneous Determination of 31 Foodborne Stimulant Drug Residues in Animal-derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with with pass-through solid phase extraction

Abstract

Abstract: Abstract: In order to solve the problems of different matrix effects and interference of foodborne doping testing in different animal-derived foods，a method was established for the determination of ten protein anabolic agents, eight glucocorticoids, and twelve diuretics in animal-derived foods by pass-through solid phase extraction -high performance liquid chromatography-tandem mass spectrometry (SPE-HPLC-MS/MS). The sample was extracted with 5% formic acid in acetonitrile, and the extract was purified by pass-through solid phase extraction before being measured by liquid chromatography-tandem mass spectrometry. The internal standard method was used for quantification. The linear relationship of 30 foodborne stimulants was good within their respective concentration ranges. The average recoveries ranged from 67.5% to 114.3%, and the relative standard deviations were between 0.7% and 16.1% (n = 6). This method has the characteristics of high sensitivity, simple operation, large detection flux and high detection efficiency, improves the measurement accuracy under different complex substrates. It can meet the detection needs of 30 kinds of foodborne stimulants in animal-derived food for sports events.

Key words: Keywords: high performance liquid chromatography-tandem mass spectrometry, animal-derived foods, protein anabolic agent, glucocorticoids, diuretics

CLC Number:

LIU Chuan Yan-Qiu CHEN. Simultaneous Determination of 31 Foodborne Stimulant Drug Residues in Animal-derived Foods by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with with pass-through solid phase extraction[J]. FOOD SCIENCE, 0, (): 0-0.

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