Abstract: Food safety incidents caused by foodborne pathogens have caused widespread concern about the increasing number of food safety accidents induced by foodborne pathogens, in order to ensure food safety, it is essential to adopt effective means of detection. The emerging CRISPR/Cas system, consisting of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated proteins (Cas), is an acquired immune system widely found in bacteria and archaea, which efficiently and specifically recognizes and cleaves exogenous nucleic acids, and is more effective in detecting pathogenic bacteria than conventional techniques. Based on the research on CRISPR/Cas biosensing system coupled with isothermal amplification technology, this paper summarizes the classification, principle, and progress of CRISPR/Cas system coupled with isothermal amplification in foodborne pathogen detection, and focuses on the prospect of its immediate detection application and the challenges it is facing, in order to provide a reference for the further development of CRISPR/Cas biosensing system.

Key words: foodborne pathogens, CRISPR/Cas system, isothermal amplification, nanomaterials, functional nucleic acids