Lipidomics profile, fatty acid distribution, and differential analysis of lipid molecules in dried shrimps (Litopenaeus vannamei)

Abstract

Abstract: This study aimed to identify lipid molecules species, composition, and the distribution of fatty acid positions in dried shrimps, as well as screening differential lipids and investigating lipid profiles changes and lipid transformation mechanism during storage using lipidomics analysis. Results showed that a total of 790 lipid molecular species belonging to 7 main classes were identified, among which glycerophospholipids were the most abundant, followed by glycerolipids and sphingolipids. In phosphatidylcholine (PC), phosphatidylethanolamine (PE), triglycerides (TG), and diglycerides (DG), saturated fatty acids were mainly located at the sn-1 position, while unsaturated fatty acids, especially oleic acid, linoleic acid, docosahexaenoic acid (DHA), and eicosapentaenoic acid (EPA), were mainly distributed at the sn-2 position. Using multivariate statistical analysis, 151 differential lipid molecules were screened, including 121 and 30 differentially abundant lipids (DALs) accumulating at high and low abundance levels, respectively. During 20 days of storage, lyso-phosphatidylcholine, lyso-phosphatidylethanolamine, and lyso-phosphatidylserine increased, while PC, PE, and phosphatidylserine decreased significantly, indicating the occurrence of lipid transformation.

Key words: Dried shrimps, Lipidomics, Fatty acids, Differential lipid molecules, Transformation

CLC Number:

TS205.1

References

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