Research progress and influencing factors on LC-MS/MS method of paralytic shellfish toxins in bivalve shellfish

Abstract

Abstract: Paralytic shellfish toxins (PSTs), which pose the greatest threat and have the widest distribution among marine biotoxins, significantly impact human health, fisheries, and marine environments. Bivalve mollusks are the primary source of PSTs ingestion for consumers. As PSTs poisoning incidents increase, global demand for PSTs detection methods in bivalve mollusks is rising. This article primarily discusses and compares the advantages and disadvantages of LC-MS/MS, a mainstream method for detecting PSTs in bivalve shellfish, focusing on PSTs physicochemical properties, pre-processing extraction and purification, instrument conditions, matrix effects, and method validation. Through comparative analysis, it is evident that 1% HAc is the most commonly used extraction reagent in liquid chromatography-based PSTs detection. GCB and HILIC are suitable for purifying and separating PSTs extracts from bivalve shellfish. Factors affecting matrix effects include endogenous interferences such as phospholipids and proteins, as well as exogenous interferences related to mobile phase composition and mass spectrometry parameters. Additionally, this article highlights the research trends in LC-MS/MS methodology, aiming to provide reference for PSTs detection in bivalve shellfish and support sustainable development and government regulation in shellfish aquaculture.

Key words: paralytic shellfish toxins, bivalve shellfish, liquid chromatography tandem mass spectrometry(LC-MS/MS), influencing factors

CLC Number:

TS254.4

Yu-Xiang DENG Hui-Hui ZHAO Zhi-Jun Tan. Research progress and influencing factors on LC-MS/MS method of paralytic shellfish toxins in bivalve shellfish[J]. FOOD SCIENCE.

References

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