Simultaneous Determination of 39 plant toxins in honey by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid phase extraction

Abstract

Abstract: To effectively assess food safety risks associated with plant toxins in honey, this study aims to establish and validate a detection method using solid-phase extraction-ultra-performance liquid chromatography-tandem mass spectrometry. The method involves extracting the sample with 0.1% formic acid water, purifying it with an HLB cartridge, detecting it through multiple reaction monitoring mode (MRM), and quantifying it using the matrix-matched external standard method. The 39 plant toxins exhibit a strong linear relationship within their concentration ranges, with average recovery rates ranging from 78.7% to 112.8% and relative standard deviations from 1.6% to 15.2% (n=6). This method offers simplicity in operation, high detection throughput, and sensitivity, making it suitable for monitoring these toxins in honey. Analysis of 36 sample batches revealed varying levels of Pyrrolizidine alkaloids(PAs) and RhodojaponinⅢ, highlighting the presence of residual phytotoxins in honey and confirming associated risks.

Key words: Solid-phase extraction(SPE), Ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS), Honey, Plant toxins

CLC Number:

LIU Chuan Yan-Qiu CHEN Hao LIN Wang luyao Wen-Lin WU. Simultaneous Determination of 39 plant toxins in honey by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid phase extraction[J]. FOOD SCIENCE, 0, (): 0-0.

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