Functional Roles and Industrialization Innovations of Perilla frutescens Active Components: From Molecular Mechanisms to Cross-Disciplinary Applications

Abstract

Abstract: Perilla frutescens (L.) Britt., a traditional medicinal-edible plant in China, exemplifies the dual value of nutritional and therapeutic benefits. Enriched with bioactive constituents including phenolic acids, fatty acids, terpenoids, polysaccharides, and flavonoids, it exhibits extensive biological activities. This review systematically analyzes the functions of different active components of Perilla frutescens and summarizes its role in antioxidant defense and cellular homeostasis maintenance, metabolism and immunity regulation, and modulation in the nervous system based on the modulation of Perilla frutescens biologically active substances on the health of the organism. From cross-domain applications, we synthesize Perilla frutescens' innovative industrial practices in functional foods, pharmaceuticals, and agricultural biocontrol, while exploring precision development technologies spanning genomics, CRISPR editing, green extraction processes, and quality control standardization systems. This review proposes multidisciplinary integration frameworks to establish scientific foundations for the characterization and industrial innovation of bioactive components in Perilla frutescens, providing a theoretical basis for their in-depth development in modern health-centric industries.

Key words: Keywords：Perilla frutescens, medicinal-edible plant, bioactive components, health efficacy, functional foods, industrial innovation

CLC Number:

TS201.2

Zheng Li Bowen Li. Functional Roles and Industrialization Innovations of Perilla frutescens Active Components: From Molecular Mechanisms to Cross-Disciplinary Applications[J]. FOOD SCIENCE.

References

[1] 于红卫. 药食同源——紫苏[J]. 河南农业, 2024, (01): 2. DOI:10.15904/j.cnki.hnny.2024.01.052
[2] 陈琳. 紫苏油微胶囊的制备及在食品中的应用研究[D]. 天津; 天津商业大学, 2014:32-34
[3] 张婉萍, 陈婕, 王恒, 等. 紫苏叶的活性成分及生物活性研究进展[J]. 食品与发酵工业, 2023, 49(20): 361-368. DOI:10.13995/j.cnki.11-1802/ts.033179
[4] LIU Jer-Yuh, CHEN Yi-Ching, LIN Chun-Hsiang, et al. Perilla frutescens Leaf Extract Inhibits Mite Major Allergen Der p 2-induced Gene Expression of Pro-Allergic and Pro-Inflammatory Cytokines in Human Bronchial Epithelial Cell BEAS-2B[J]. PLOS ONE, 2013, 8(10): e77458. DOI:10.1371/journal.pone.0077458
[5] SUN-WATERHOUSE Dong-Xiao, CHEN Xiao-Yu, LIU Zhen-Hua, et al. Transformation from traditional medicine-food homology to modern food-medicine homology[J]. Food & Medicine Homology, 2024, 1(1): 9420014. DOI:10.26599/FMH.2024.9420014
[6] 曹娅, 张金龙, 王强. 紫苏活性成分及其生物功能的研究进展[J]. 中国食物与营养, 2021, 27(08): 42-49. DOI:10.19870/j.cnki.11-3716/ts.20210617.001
[7] 张琛武, 郭佳琪, 郭宝林. 紫苏中酚酸类成分研究进展[J]. 中国现代中药, 2017, 19(11): 1651-1658. DOI:10.13313/j.issn.1673-4890.2017.11.031
[8] 贾凯慧, 李占君, 姚远, 等. 超声辅助提取紫苏粕迷迭香酸工艺优化及其抗氧化能力[J]. 森林工程, 2022, 38(04): 89-97.
[9] LEE Kyeong-Ryeol, LEE Yongjik, KIM Eun-Ha, et al. Functional identification of oleate 12-desaturase and ω-3 fatty acid desaturase genes from Perilla frutescens var. frutescens[J]. Plant Cell Reports, 2016, 35(12): 2523-2537. DOI:10.1007/s00299-016-2053-4
[10] HAO Xu-Ting, PENG Rui, GUAN Miao, et al. The food and medicinal homological resources benefiting patients with hyperlipidemia: categories, functional components, and mechanisms[J]. Food & Medicine Homology, 2024, 1(2): 9420003. DOI:10.26599/FMH.2024.9420003
[11] KANGWAN Napapan, PINTHA Komsak, KHANAREE Chakkrit, et al. Anti-inflammatory effect of Perilla frutescens seed oil rich in omega-3 fatty acid on dextran sodium sulfate-induced colitis in mice[J]. Research in Pharmaceutical Sciences, 2021, 16(5): 464-473. DOI:10.4103/1735-5362.323913
[12] 李方, 张梦飞, 王宪龄. 红花籽油、紫苏籽油调和油干预高脂血症的可行性[J]. 中医学报, 2023, 38(06): 1240-1245. DOI:10.16368/j.issn.1674-8999.2023.06.206
[13] 王晓辉. 紫苏化学成分和抗肿瘤的研究[D]. 吉林; 吉林大学, 2016:15-18
[14] CUI Xiaoyan, GOU Zhongyong, FAN Qiuli, et al. Effects of dietary perilla seed oil supplementation on lipid metabolism, meat quality, and fatty acid profiles in Yellow-feathered chickens[J]. Poultry Science, 2019, 98(11): 5714-5723. DOI:10.3382/ps/pez358
[15] 刘本同, 温贺, 秦玉川, 等. 制油工艺对紫苏油品质的影响研究[J]. 中国粮油学报, 2022, 37(04): 128-134.
[16] 闫慧杰, 蔺永刚, 努尔买买提, 等. 紫苏的药理活性及其在动物饲料中的应用进展[J]. 饲料研究, 2023, 46(24): 143-147. DOI:10.13557/j.cnki.issn1002-2813.2023.24.028
[17] 张良琦, 李文姣, 肖美凤. 紫苏不同部位活性成分比较及其药理作用研究进展[J]. 中国中药杂志, 2023, 48(24): 6551-6571. DOI:10.19540/j.cnki.cjcmm.20230813.201
[18] ERHUNMWUNSEE Famous, PAN Chao, YANG Kunlong, et al. Recent development in biological activities and safety concerns of perillaldehyde from perilla plants: A review[J]. Critical Reviews in Food Science and Nutrition, 2022, 62(23): 6328-6340. DOI:10.1080/10408398.2021.1900060
[19] 刘子坤, 尹贺, 杨安皓, 等. 紫苏籽多糖分离纯化及抗肿瘤活性[J]. 食品科学, 2022, 43(15): 158-165.
[20] ZHU Ling, GUAN Lijun, WANG Kunlun, et al. Recent trends in extraction, purification, structural characterization, and biological activities evaluation of Perilla frutescens (L.) Britton polysaccharide[J]. Frontiers in Nutrition, 2024, 11. DOI:10.3389/fnut.2024.1359813
[21] 王士博, 孔令雪, 刘金娟. 紫苏多糖体外抗氧化活性及对肝细胞氧化损伤的保护作用[J]. 中国油脂, 2024, 49(09): 120-127. DOI:10.19902/j.cnki.zgyz.1003-7969.230284
[22] URUSHIMA Hayato, NISHIMURA Junichi, MIZUSHIMA Tsunekazu, et al. Perilla frutescens extract ameliorates DSS-induced colitis by suppressing proinflammatory cytokines and inducing anti-inflammatory cytokines[J]. American Journal of Physiology-Gastrointestinal and Liver Physiology, 2014, 308(1): G32-G41. DOI:10.1152/ajpgi.00294.2014
[23] 尹丽, 孟学林. 正交试验法优化紫苏叶中总黄酮提取工艺及体外抗氧化活性研究[J]. 饲料研究, 2024, 47(04): 99-103. DOI:10.13557/j.cnki.issn1002-2813.2024.04.018
[24] 唐飞, 冯五文, 敖慧. 紫苏叶药理作用研究进展[J]. 成都中医药大学学报, 2021, 44(04): 93-97+112. DOI:10.13593/j.cnki.51-1501/r.2021.04.093
[25] 郝熙, 李伟, 张海鹏, 等. 酶辅助超声优化提取紫苏籽多酚及其抗氧化和酶抑制活性[J]. 中国调味品, 2025, 50(02): 112-120.
[26] BYUN Eui-Baek, CHO Eun-Ji, KIM Yi-Eun, et al. Neuroprotective effect of polysaccharide separated from Perilla frutescens Britton var. acuta Kudo against H2O2-induced oxidative stress in HT22 hippocampus cells[J]. Bioscience, Biotechnology, and Biochemistry, 2018, 82(8): 1344-1358. DOI:10.1080/09168451.2018.1460572
[27] 吴晨. 紫苏油的抗衰老作用及其机制研究[D]. 武汉; 武汉工业学院, 2012:9-14
[28] 刘浩. 紫苏不同部位营养品质及抗氧化能力的比较研究[J]. 中国食品添加剂, 2022, 33(10): 237-244. DOI:10.19804/j.issn1006-2513.2022.10.029
[29] LEE Ah Young, WU Ting-Ting, HWANG Bo Ra, et al. The Neuro-Protective Effect of the Methanolic Extract of Perilla frutescens var. japonica and Rosmarinic Acid against H2O2-Induced Oxidative Stress in C6 Glial Cells[J]. Biomolecules & Therapeutics, 2016, 24: 338 - 345. DOI:10.4062/biomolther.2015.135
[30] 王颖. 紫苏异酮对肝癌细胞的放疗增敏及其作用机制探讨[D]. 广州; 南方医科大学, 2013:8-12
[31] KWAK Youngeun, JU Jihyeung. Inhibitory activities of Perilla frutescens britton leaf extract against the growth, migration, and adhesion of human cancer cells[J]. Nutr Res Pract, 2015, 9(1): 11-16. DOI:10.4162/nrp.2015.9.1.11
[32] 齐欣, 路欣彤, 林长青, 等. 紫苏叶提取物对1型糖尿病小鼠降血糖作用[J]. 食品与机械, 2021, 37(11): 153-158. DOI:10.13652/j.issn.1003-5788.2021.11.027
[33] 马海英, 张玉杰. 紫苏籽油与亚麻籽油合用安全性及降血脂作用研究[J]. 现代预防医学, 2022, 49(23): 4300-4305+4378. DOI:10.20043/j.cnki.MPM.202207127
[34] 白宝清, 贾槐旺, 张锦华, 等. 紫苏籽粕抗氧化肽的纯化、鉴定及降血脂功效研究[J]. 中国粮油学报, 2022, 37(07): 92-101.
[35] WANG Feng, ZHU Hangju, HU Mingyuan, et al. Perilla Oil Supplementation Improves Hypertriglyceridemia and Gut Dysbiosis in Diabetic KKAy Mice[J]. Molecular Nutrition & Food Research, 2018, 62(24): 1800299. DOI:10.1002/mnfr.201800299
[36] VON SAMSON-HIMMELSTJERNA G. 紫苏黄酮抗炎活性研究及口服液制备[D]. 吉林; 吉林农业大学, 2022:3-7
[37] PARK Deung Dae, YUM Hye-Won, ZHONG Xiancai, et al. Perilla frutescens Extracts Protects against Dextran Sulfate Sodium-Induced Murine Colitis: NF-κB, STAT3, and Nrf2 as Putative Targets[J]. Frontiers in Pharmacology, 2017, 8. DOI:10.3389/fphar.2017.00482
[38] CHANG Hui-Hsiang, CHEN Chin-Shun, LIN Jin-Yuarn. Dietary Perilla Oil Inhibits Proinflammatory Cytokine Production in the Bronchoalveolar Lavage Fluid of Ovalbumin-Challenged Mice[J]. Lipids, 2008, 43(6): 499-506. DOI:10.1007/s11745-008-3171-8
[39] 赵奕栋, 孙文豪, 陈天源, 等. 紫苏叶有效成分药理作用研究进展[J]. 江苏中医药, 2022, 54(08): 79-82. DOI:10.19844/j.cnki.1672-397X.2022.08.024
[40] CHEN Miaw-Ling, WU Chi-Heng, HUNG Li-Shiuan, et al. Ethanol Extract of Perilla frutescens Suppresses Allergen-Specific Th2 Responses and Alleviates Airway Inflammation and Hyperreactivity in Ovalbumin-Sensitized Murine Model of Asthma[J]. Evidence-Based Complementary and Alternative Medicine, 2015, 2015(1): 324265. DOI:10.1155/2015/324265
[41] KAMEI Rikiya, FUJIMURA Takashi, MATSUDA Miki, et al. A flavanone derivative from the Asian medicinal herb (Perilla frutescens) potently suppresses IgE-mediated immediate hypersensitivity reactions[J]. Biochemical and Biophysical Research Communications, 2017, 483(1): 674-679. DOI:10.1016/j.bbrc.2016.12.083
[42] 王光慧, 余帅, 王梓懿, 等. 紫苏精油和毛地黄鼠尾草精油的挥发性成分及生物活性[J]. 微生物学通报, 2024, 51(09): 3409-3421. DOI:10.13344/j.microbiol.china.230990
[43] 胡振阳. 紫苏精油对黄曲霉抑制作用与机理研究[D]. 南京; 南京财经大学, 2021:6-10
[44] 王喜龙, 张丽蓉, 欧晓彬, 等. 响应面法优化紫苏叶中抑菌成分的提取工艺[J]. 化学与生物工程, 2024, 41(12): 36-43.
[45] 余雨婷, 张彦, 张迎, 等. 紫苏体外对代谢综合征相关四种酶抑制作用的研究[J]. 中国食品添加剂, 2022, 33(04): 63-71. DOI:10.19804/j.issn1006-2513.2022.04.009
[46] KIM Hye-Kyeong, CHOI Sungwon, CHOI Haymie. Suppression of hepatic fatty acid synthase by feeding α-linolenic acid rich perilla oil lowers plasma triacylglycerol level in rats[J]. The Journal of Nutritional Biochemistry, 2004, 15(8): 485-492. DOI:10.1016/j.jnutbio.2004.02.010
[47] 陈雪梅, 胡博, 唐晓姝, 等. 紫苏籽油对小鼠肠道微生物及抗氧化能力的影响[J]. 食品与发酵工业, 2024, 50(16): 271-277. DOI:10.13995/j.cnki.11-1802/ts.037422
[48] WANG Jiankang, HE Yutong, YU Deqing, et al. Perilla oil regulates intestinal microbiota and alleviates insulin resistance through the PI3K/AKT signaling pathway in type-2 diabetic KKAy mice[J]. Food and Chemical Toxicology, 2020, 135: 110965. DOI:10.1016/j.fct.2019.110965
[49] 杜艳, 殷丽君, 朱巧梅, 等. 紫苏油的药理活性及其缓释技术研究进展[J]. 河南工业大学学报(自然科学版), 2016, 37(04): 114-117+123. DOI:10.16433/j.cnki.issn1673-2383.2016.04.021
[50] LEE Hsiu-Chuan, KO Hsiang-Kai, HUANG Brian E. T. G., et al. Antidepressant-like effects of Perilla frutescens seed oil during a forced swimming test[J]. Food & Function, 2014, 5(5): 990-996. DOI:10.1039/C3FO60717H
[51] LEE Jinwoo, PARK Sunmin, LEE Ju-Young, et al. Improved spatial learning and memory by perilla diet is correlated with immunoreactivities to neurofilament and α-synuclein in hilus of dentate gyrus[J]. Proteome Science, 2012, 10(1): 72. DOI:10.1186/1477-5956-10-72
[52] LEE Ah Young, LEE Myoung Hee, LEE Sanghyun, et al. Alpha-Linolenic Acid from Perilla frutescens var. japonica Oil Protects Aβ-Induced Cognitive Impairment through Regulation of APP Processing and Aβ Degradation[J]. Journal of Agricultural and Food Chemistry, 2017, 65(49): 10719-10729. DOI:10.1021/acs.jafc.7b03941
[53] CHOI Soo Jung, OH Seung Sang, KIM Cho Rong, et al. Perilla frutescens Extract Ameliorates Acetylcholinesterase and Trimethyltin Chloride-Induced Neurotoxicity[J]. Journal of Medicinal Food, 2015, 19(3): 281-289. DOI:10.1089/jmf.2015.3540
[54] ZHAO Gang, YAO-YUE Chen, QIN Guo-Wei, et al. Luteolin from Purple Perilla mitigates ROS insult particularly in primary neurons[J]. Neurobiology of Aging, 2012, 33(1): 176-186. DOI:10.1016/j.neurobiolaging.2010.02.013
[55] SONG Yichen, SUN Ruoxi, JI Zhouye, et al. Perilla aldehyde attenuates CUMS-induced depressive-like behaviors via regulating TXNIP/TRX/NLRP3 pathway in rats[J]. Life Sciences, 2018, 206: 117-124. DOI:10.1016/j.lfs.2018.05.038
[56] JI Wei-Wei, LI Rui-Peng, LI Meng, et al. Antidepressant-like effect of essential oil of Perilla frutescens in a chronic, unpredictable, mild stress-induced depression model mice[J]. Chinese Journal of Natural Medicines, 2014, 12(10): 753-759. DOI:10.1016/S1875-5364(14)60115-1
[57] ZHONG Yu, ZHENG Qin, HU Pengyi, et al. Sedative and hypnotic effects of Perilla frutescens essential oil through GABAergic system pathway[J]. Journal of Ethnopharmacology, 2021, 279: 113627. DOI:10.1016/j.jep.2020.113627
[58] 吴文龙, 曾少葵, 黄楚欣, 等. 紫苏复合调味酱加工工艺的研究[J]. 中国调味品, 2022, 47(03): 102-106.
[59] 黄丽琪, 胡传峰, 鲁怡婷, 等. 紫苏精油和姜汁对烤武昌鱼的风味影响[J]. 现代食品科技, 2024, 40(07): 193-202. DOI:10.13982/j.mfst.1673-9078.2024.7.0718
[60] 马明月, 姜宏宇, 张华. 紫苏油微胶囊的性质与体外模拟消化研究[J]. 中国粮油学报, 2022, 37(05): 102-108.
[61] 唐森, 李孟玲, 陈显玲, 等. 紫苏精油壳聚糖复合膜的制备及其对鲜切雪莲果的保鲜效果研究[J]. 粮食与油脂, 2023, 36(09): 61-65+83.
[62] 李紫薇, 车萍, 张颖颖, 等. 紫苏化学成分及药理作用研究进展[J]. 山东化工, 2024, 53(03): 111-114. DOI:10.19319/j.cnki.issn.1008-021x.2024.03.022
[63] 杨慧, 马培, 林明宝, 等. 紫苏叶化学成分、抗炎作用及其作用机制研究进展[J]. 中国药理学与毒理学杂志, 2017, 31(03): 279-286.
[64] 郭雪红. 中药紫苏药理及临床研究新进展[J]. 天津药学, 2016, 28(02): 70-73.
[65] BAE Jung-Soo, HAN Mira, SHIN Hee Soon, et al. Perilla frutescens leaves extract ameliorates ultraviolet radiation-induced extracellular matrix damage in human dermal fibroblasts and hairless mice skin[J]. Journal of Ethnopharmacology, 2017, 195: 334-342. DOI:10.1016/j.jep.2016.11.039
[66] LEE Hana, SUNG Jeehye, KIM Younghwa, et al. Protective Effects of Unsaponifiable Matter from Perilla Seed Meal on UVB-induced Damages and the Underlying Mechanisms in Human Skin Fibroblasts[J]. Antioxidants, 2019, 8(12): 644. DOI:10.3390/antiox8120644
[67] 曹扬远, 黄桂珍, 郑佛荣. 紫苏挥发油的提取及其应用研究进展[J]. 海峡药学, 2024, 36(07): 29-32.
[68] 袁康, 胡振阳, 卢臣, 等. 紫苏精油对两种霉菌的抑菌活性效果研究[J]. 粮食科技与经济, 2019, 44(11): 85-89. DOI:10.16465/j.gste.cn431252ts.20191121
[69] YOU Chun-xue, WANG Ying, ZHANG Wen-juan, et al. Chemical constituents and biological activities of the Purple Perilla essential oil against Lasioderma serricorne[J]. Industrial Crops and Products, 2014, 61: 331-337. DOI:10.1016/j.indcrop.2014.07.021
[70] 宋宇. 历史时期紫苏的种植与利用研究[J]. 信阳农林学院学报, 2020, 30(04): 101-105+109. DOI:10.16593/j.cnki.41-1433/s.2020.04.023
[71] 郭旭, 田荣荣, 张东. 紫苏油的提取工艺和药理功能研究进展[J]. 粮油食品科技, 2021, 29(05): 120-130. DOI:10.16210/j.cnki.1007-7561.2021.05.016
[72] ZHOU Qi, HU Zhenyang, DU Lihui, et al. Inhibition of Enterococcus faecalis Growth and Cell Membrane Integrity by Perilla frutescens Essential Oil[J]. Foodborne Pathogens and Disease, 2020, 17(9): 547-554. DOI:10.1089/fpd.2019.2771
[73] YOU Chun Xue, YANG Kai, WU Yan, et al. Chemical composition and insecticidal activities of the essential oil of Perilla frutescens (L.) Britt. aerial parts against two stored product insects[J]. European Food Research and Technology, 2014, 239(3): 481-490. DOI:10.1007/s00217-014-2242-8
[74] TONG Wei, KWON Soon-Jae, LEE Jeongsoo, et al. Gene set by de novo assembly of Perilla species and expression profiling between P. frutescens (L.) var. frutescens and var. crispa[J]. Gene, 2015, 559(2): 155-163. DOI:10.1016/j.gene.2015.01.028
[75] JIANG Tao, GUO Kunyuan, LIU Lingdi, et al. Integrated transcriptomic and metabolomic data reveal the flavonoid biosynthesis metabolic pathway in Perilla frutescens (L.) leaves[J]. Scientific Reports, 2020, 10(1): 16207. DOI:10.1038/s41598-020-73274-y
[76] FUKUSHIMA Atsushi, NAKAMURA Michimi, SUZUKI Hideyuki, et al. High-Throughput Sequencing and De Novo Assembly of Red and Green Forms of the Perilla frutescens var. crispa Transcriptome[J]. PLOS ONE, 2015, 10(6): e0129154. DOI:10.1371/journal.pone.0129154
[77] WANG Xin, MIAO Yu-Hang, ZHAO Xiao-Min, et al. Perspectives on organ-on-a-chip technology for natural products evaluation[J]. Food & Medicine Homology, 2024, 1(2): 9420013. DOI:10.26599/FMH.2024.9420013
[78] KIM Kyung-Hwan, LEE Yeon-Hee, KIM Donghern, et al. Agrobacterium-mediated genetic transformation of Perilla frutescens[J]. Plant Cell Reports, 2004, 23(6): 386-390. DOI:10.1007/s00299-004-0825-8
[79] 于海鑫. 紫苏主要成分分析及紫苏粉的研制[D]; 东北农业大学, 2019:15-17
[80] ZHAO Qiaoli, WANG Lifeng, HONG Xin, et al. Structural and functional properties of perilla protein isolate extracted from oilseed residues and its utilization in Pickering emulsions[J]. Food Hydrocolloids, 2021, 113: 106412. DOI:10.1016/j.foodhyd.2020.106412
[81] SINGH Naseeb, KAUR Simardeep, MAHORE Vijay, et al. Development of deep learning-based mobile application for predicting in-situ habitat suitability of Perilla frutescens L. in real-time[J]. Smart Agricultural Technology, 2024, 8: 100508. DOI:10.1016/j.atech.2024.100508
[82] 付晨青, 郭潇潇, 刘徐冬雨, 等. 贮藏温度对鲜食紫苏采后品质的影响及货架期预测模型的构建[J]. 北方园艺, 2024, (02): 86-93.
[83] KIM Hyun Uk, LEE Kyeong-Ryeol, SHIM Donghwan, et al. Transcriptome analysis and identification of genes associated with ω-3 fatty acid biosynthesis in Perilla frutescens (L.) var. frutescens[J]. BMC Genomics, 2016, 17(1): 474. DOI:10.1186/s12864-016-2805-0
[84] LIAO BingNan, HAO YouJin, LU JunXing, et al. Transcriptomic analysis of Perilla frutescens seed to insight into the biosynthesis and metabolic of unsaturated fatty acids[J]. BMC Genomics, 2018, 19(1): 213. DOI:10.1186/s12864-018-4595-z
[85] 朴颖, 费宏扬, 权海燕. 紫苏叶提取物对肥胖小鼠的影响及作用机制[J]. 中华中医药杂志, 2017, 32(09): 3992-3996.
[86] LI Na, ZHANG Zhi-Jun, LI Xiao-Jun, et al. Microcapsules biologically prepared using (L.) Britt. essential oil and their use for extension of fruit shelf life[J]. Journal of the Science of Food and Agriculture, 2018, 98(3): 1033-1041. DOI:10.1002/jsfa.8552
[87] WANG Hongsu, GUO Ling, LIU Lu, et al. Composite chitosan films prepared using nisin and Perilla frutescense essential oil and their use to extend strawberry shelf life[J]. Food Bioscience, 2021, 41: 101037. DOI:10.1016/j.fbio.2021.101037
[88] LU Na, BERNARDO Emmanuel L., TIPPAYADARAPANICH Chayanit, et al. Growth and Accumulation of Secondary Metabolites in Perilla as Affected by Photosynthetic Photon Flux Density and Electrical Conductivity of the Nutrient Solution[J]. Frontiers in Plant Science, 2017, 8. DOI:10.3389/fpls.2017.00708
[89] 路广平, 王宏凯, 刘众, 等. 三种栽培模式对紫苏产量性状及产量的影响[J]. 西北园艺, 2024, (05): 61-63.
[90] 廖楠汐, 石德志, 肖莲莲, 等. 基于指标成分测定及指纹图谱模式识别的紫苏叶产地差异性研究[J]. 中草药, 2024, 55(18): 6355-6362.
[91] TIAN Jun, ZENG Xiaobin, ZHANG Shu, et al. Regional variation in components and antioxidant and antifungal activities of Perilla frutescens essential oils in China[J]. Industrial Crops and Products, 2014, 59: 69-79. DOI:10.1016/j.indcrop.2014.04.048
[92] YU Dai-xin, QU Cheng, XU Jia-yi, et al. Rapid discrimination and quantification of chemotypes in Perillae folium using FT-NIR spectroscopy and GC–MS combined with chemometrics[J]. Food Chemistry: X, 2024, 24: 101881. DOI:10.1016/j.fochx.2024.101881
[93] 许兰杰, 梁慧珍, 余永亮, 等. 我国紫苏种质资源遗传多样性研究进展[J]. 安徽农业科学, 2020, 48(11): 19-20+25.
[94] 张良琦, 陈凡, 张子桉, 等. 基于网络药理学和UPLC指纹图谱的紫苏质量标志物(Q-Marker)预测[J]. 天然产物研究与开发, 2024, 36(10): 1800-1812. DOI:10.16333/j.1001-6880.2024.10.016
[95] 刘鸣茹, 朱永平, 陈莎. 二倍体紫苏CYP71基因家族的鉴定和生物信息学分析[J]. 中国实验方剂学杂志, 2023, 29(11): 160-170. DOI:10.13422/j.cnki.syfjx.20230315
[96] KIM Jae Kwang, PARK Soo-Yun, NA Jong-Kuk, et al. Metabolite Profiling Based on Lipophilic Compounds for Quality Assessment of Perilla (Perilla frutescens) Cultivars[J]. Journal of Agricultural and Food Chemistry, 2012, 60(9): 2257-2263. DOI:10.1021/jf204977x
[97] WANG Zhen-xing, JIN Ximeng, ZHANG Xuechun, et al. From Function to Metabolome: Metabolomic Analysis Reveals the Effect of Probiotic Fermentation on the Chemical Compositions and Biological Activities of Perilla frutescens Leaves[J]. Frontiers in Nutrition, 2022, 9. DOI:10.3389/fnut.2022.933193
[98] 丁友芳. 紫苏种子质量标准及其醇提物的生物活性研究[D]. 北京; 北京林业大学, 2012:92-94
[99] MüLLER-WALDECK Franziska, SITZMANN Judith, SCHNITZLER Wilfried H., et al. Determination of toxic perilla ketone, secondary plant metabolites and antioxidative capacity in five Perilla frutescens L. varieties[J]. Food and Chemical Toxicology, 2010, 48(1): 264-270. DOI:10.1016/j.fct.2009.10.009
[100] YU Hua, QIU Jian-Feng, MA Li-Juan, et al. Phytochemical and phytopharmacological review of Perilla frutescens L. (Labiatae), a traditional edible-medicinal herb in China[J]. Food and Chemical Toxicology, 2017, 108: 375-391. DOI:10.1016/j.fct.2016.11.023
[101] 李学红, 陆勇, 郝晓萍, 等. 环糊精稳定紫苏籽油Pickering乳液的制备及其稳定性[J]. 中国粮油学报, 2019, 34(12): 59-64.
[102] ZHANG Shun, MA Dong-Wei, YANG Xin, et al. The effect of chitosan on blood lipids in patients with hypercholesterolemia and hyperlipidemia: a systematic review and meta-analysis[J]. Food & Medicine Homology, 2025. DOI:10.26599/FMH.2025.9420061
[103] WANG Debao, SUN Zhilan, SUN Jinyue, et al. Preparation and characterization of polylactic acid nanofiber films loading Perilla essential oil for antibacterial packaging of chilled chicken[J]. International Journal of Biological Macromolecules, 2021, 192: 379-388. DOI:10.1016/j.ijbiomac.2021.09.190
[104] NGUYEN Thi Kim Loan, OH Myung-Min. Physiological and biochemical responses of green and red perilla to LED-based light[J]. Journal of the Science of Food and Agriculture, 2021, 101(1): 240-252. DOI:10.1002/jsfa.10636
[105] 梁晶春, 梁洁, 黄团心, 等. 紫苏质量标志物预测分析[J]. 中华中医药学刊, 2023, 41(04): 131-134. DOI:10.13193/j.issn.1673-7717.2023.04.026
[106] 刘振杰, 丘海芯, 甘金月, 等. 紫苏叶HPLC指纹图谱建立及其抗流感病毒活性谱效关系研究[J]. 中草药, 2024, 55(01): 287-296.
[107] SUO Ke-Ke, LI Xue, LIU Xin, et al. From environment to environmental adaptation: Environmental perspectives on the study of food and medicine homology[J]. Food & Medicine Homology, 2025. DOI:10.26599/FMH.2026.9420082
[108] SAINI Ramesh Kumar, KEUM Young-Soo, RENGASAMY Kannan R. R. Profiling of nutritionally important metabolites in green/red and green perilla (Perilla frutescens Britt.) cultivars: A comparative study[J]. Industrial Crops and Products, 2020, 151: 112441. DOI:10.1016/j.indcrop.2020.112441
[109] 邢聪会, 杨光伟, 徐芳, 等. 东紫苏精油急性毒性评价[J]. 实用预防医学, 2025, 32(01): 54-59.