Degradation of Aflatoxin B1 and Zearalenone in Vegetable Oils Using MOF-Immobilized Laccase: A Comparative Study

Abstract

Abstract: Aflatoxin B1 (AFB1) and zearalenone (ZEN) are major contaminants in vegetable oils. Conventional physical and chemical detoxification methods demonstrate limited efficacy. Consequently, developing green, efficient, and safe mycotoxin removal technologies is critically needed. This study employed Fe-MOFs as carriers to immobilize laccase (Lac) via in-situ encapsulation, synthesizing a Lac@Fe-BTC biocomposite. Then the removal efficacy and underlying mechanisms of this composite for AFB1 and ZEN in edible oils were then investigated.Results indicated that Laccase immobilization on MOFs enhanced thermal stability and achieved AFB1 and ZEN removal rates of 71.64% and 60.98% in peanut oil, respectively, significantly exceeding those of free laccase.Furthermore, structural characterization of the mycotoxins and their degradation products, alongside zebrafish toxicity evaluation, demonstrated a significant reduction in their toxicity.Compared to free laccase, the immobilized enzyme demonstrated superior efficiency, stability, and reusability for mycotoxin removal. Therefore, this approach exhibits promising potential for mycotoxin decontamination applications.

Key words: Keywords: Immobilized laccase, Metal-organic frameworks (MOFs), Peanut oil, Mycotoxins degradation, Safety

CLC Number:

TS201. 2

Meng-Yao WANG. Degradation of Aflatoxin B1 and Zearalenone in Vegetable Oils Using MOF-Immobilized Laccase: A Comparative Study[J]. FOOD SCIENCE.

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