Determination of 14 Mono-phenols in Dry Red Wines by HPLC

Abstract

Abstract: The contents of 14 mono-phenols in six Cabernet Sauvignon dry red wines with different aging times or different geographical origins of grape were determined using a HPLC method. The chromatographic separation was performed on a ZORBAX SB-C18 column at 30 ℃ with an acetonitrile/acetic acid/water mixture as a mobile phase at a flow rate of 1.0 mL/min and the detection wavelength was set at 280 nm. The contents of 14 mono-phenols in Cabernet Sauvignon wine varied with geographical origin of grape, and higher contents of gallic acid, catechin, and epicatechin were found in all the six wine samples. Cabernet Sauvignon wines with different aging times from the same region differed in their mono-phenols content as well. The contents of several major mono-phenols decreased with increasing aging time, while a few others exhibited an opposite change.

Key words: HPLC, wine, mono-phenols, aging time, origin

CLC Number:

TS201.2

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Determination of 14 Mono-phenols in Dry Red Wines by HPLC

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