Purification and Antioxidant Activity of Polysaccharides from Pleurotus ostreatus Mycelium

doi:10.7506/spkx1002-6630-201022026

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 124-128.doi: 10.7506/spkx1002-6630-201022026

• Processing Technology • Previous Articles Next Articles

Purification and Antioxidant Activity of Polysaccharides from Pleurotus ostreatus Mycelium

CAO Xiang-yu，LIU Jian-li*，LU Xiu-li，LOU Hong，HUO Ya-peng

(School of Life Science, Liaoning University, Shenyang 110036, China)

Received:2010-08-02 Revised:2010-10-28 Online:2010-11-25 Published:2010-12-29
Contact: LIU Jian-li E-mail:jianliliu@yahoo.com.cn

Abstract

Abstract:

Crude polysaccharides were extracted from Pleurotus ostreatus mycelium by cellulase. Optimal extraction conditions were explored to evaluate the effects of enzyme dosage, extraction temperature, extraction time and pH on extraction rate of polysaccharides through orthogonal experiments. Crude polysaccharides were purified with DEAE-32 and Sephadex G100 column chromatography. The purity of polysaccharides from Pleurotus ostreatus mycelium was determined by UV scanning and cellulose acetate electrophoresis. Antioxidant activity of polysccharides was evaluated by the scavenging capability of surperoxide anion and hydroxyl free radicals and the inhibition rate of erythrocyte hemolysis. Results indicated that the optimal extraction conditions for preparing polysaccharides from Pleurotus ostreatus mycelium by cellulase were enzyme dosage of 1.0%, extraction temperature of 55 ℃, extraction time of 2 h and pH 5.3. The extraction rate was up to (4.91 ± 0.15)% under the optimal extraction conditions. The purified polysaccharides from Pleurotus ostreatus mycelium revealed antioxidant activity such as scavenging capability of free radicals and suppression of erythrocyte hemolysis in a dosage-dependent manner. Therefore, the polysaccharides from Pleurotus ostreatus mycelium have strong antioxidant activity in vitro in a certain concentration range.

Key words: Pleurotus ostreatus mycelium, polysaccharides, purification, antioxidation

CLC Number:

Q939.9

CAO Xiang-yu，LIU Jian-li*，LU Xiu-li，LOU Hong，HUO Ya-peng. Purification and Antioxidant Activity of Polysaccharides from Pleurotus ostreatus Mycelium[J]. FOOD SCIENCE, 2010, 31(22): 124-128.

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Purification and Antioxidant Activity of Polysaccharides from Pleurotus ostreatus Mycelium

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