Comparison of DNA Extraction Methods for Transgenic Rice

doi:10.7506/spkx1002-6630-201022100

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (22): 445-449.doi: 10.7506/spkx1002-6630-201022100

• Analysis & Detection • Previous Articles Next Articles

Comparison of DNA Extraction Methods for Transgenic Rice

SONG Shang-xin，XIAO Hong-mei，GAO Feng，ZHOU Guang-hong*，QIU Liang-yan

Key Laboratory of Meat Processing and Quality Control, Ministry of Education, Key Laboratory of Agricultural and Animal Products
Processing and Quality Control, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China

Received:2010-03-08 Revised:2010-11-03 Online:2010-11-25 Published:2010-12-29
Contact: ZHOU Guang-hong E-mail:ghzhou@njau.edu.cn

Abstract

Abstract:

Objective: To establish a simple, fast and effective DNA extraction method from transgenic rice. Methods: DNA extraction methods including traditional CTAB method, improved alkali-treated method and boiling-treated method for DNA extraction from transgenic brown rice, non-transgenic brown rice and transgenic milled rice were compared to screen the optimal extraction method. Results: The integrity, purity and PCR result of template DNA extracted by improved alkali-treated method were similar with the DNA extracted by traditional CTAB method. Meanwhile, compared with traditional CTAB method, improved alkali-treated method had higher DNA extraction rate, less consuming time and lower cost. Conclusion: Improved alkalitreated method can replace traditional CTAB method as DNA extraction method for the detection of transgenic rice.

Key words: rice, DNA extraction, improved alkali-treated method, detection of transgene

CLC Number:

S511

SONG Shang-xin，XIAO Hong-mei，GAO Feng，ZHOU Guang-hong*，QIU Liang-yan. Comparison of DNA Extraction Methods for Transgenic Rice[J]. FOOD SCIENCE, 2010, 31(22): 445-449.

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Comparison of DNA Extraction Methods for Transgenic Rice

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