FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (18): 329-332.doi: 10.7506/spkx1002-6630-201018078

• Analysis & Detection • Previous Articles     Next Articles

Rapid Determination of Feruloylated Oligosaccharides during Production

ZHAO Yang-yang1,OU Shi-yi1,*,LIN Qi-ling1,2,LIN Hai-wei1   

  1. 1. Department of Food Science and Engineering, Jinan University, Guangzhou 510632, China;
    2. College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510641, China
  • Received:2010-06-09 Revised:2010-08-19 Online:2010-09-25 Published:2010-12-29
  • Contact: OU Shi-yi1 E-mail:tosy@jnu.edu.cn

Abstract:

In this work, feruloylated oligosaccharides were prepared from each of three raw materials, namely, wheat bran, corn cob and corn bran, by enzymatic hydrolysis. A rapid method for determining feruloylated oligosaccharides was developed and verified by high performance liquid chromatography. Non-feruloylated oligosaccharides were removed by Amberlite resin chromatography, and the purified feruloylated oligosaccharide could be determined by using dual wavelength (DW) spectrophotometry (345 nm and 375 nm) coupled with the determination of reducing sugars after acidolysis.

Key words: feruloylated oligosaccharides, ferulic acid, determination, dual wavelength spectrophotometry, highperformance liquid chromatography

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