FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (17): 120-123.doi: 10.7506/spkx1002-6630-201017027

• Basic Research • Previous Articles     Next Articles

Isolation, Purification and Structural Characterization of a Polysaccharide Fraction from Stem Tuber of Bletilla striata, Named BSPI-A

WANG Bo，XU Sha，HUANG Lin-juan，WANG Zhong-fu*   

  1. Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory
    of Biotechnology, College of Life Science, Northwest University, Xi an 710069, China
  • Received:2009-12-28 Revised:2010-08-03 Online:2010-09-15 Published:2010-12-29
  • Contact: WANG Zhong-fu E-mail:wangzhf@nwu.edu.cn

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Abstract:

The crude polysaccharide extract, named CBSP, from the stem tubers of Bletilla striata was purified/fractionated by DEAE-cellulose column chromatography, and two fractions were obtained and they were named BSPI and BSPII. BSPI was further purified by Bio-Gel P-300 column chromatography, and a sub-fraction was obtained and it was named BSPI-A. HPGPC analysis showed that the molecular weigh of BSPI-A was more than 4.0 × 105 D. By means of IR spectroscopy, gas chromatography (GC), GC coupled to mass spectrometry (GC-MS) and methylation analysis, the polysaccharide fraction was characterized structurally, and the results indicated that BSPI-A was a linear polysaccharide and that the backbone was composed of 1,4-linked mannose (Man) and 1,4-linked glucose (Glc), with a molar ratio of 8.09:1.

Key words: Bletilla striata, polysaccharide, isolation and purification, structural characterization

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