Abstract:

ERIC-PCR technique was used to analyze the phylogenetic relationships among 12 kinds of Pleurotus pulmonarius strains. The results indicated that the amplified product bands of ERIC-PCR were mainly distributed in a range from 150 to 3500 bp with good reproducibility and high polymorphism. The number of amplified bands varied from 9 to 16 for each strain. Cluster analysis revealed 79% similarity and 4 distinctive clusters among 12 strains. Based on the close phylogenetic relationship, MY Gray and MY White were designated as the first cluster, and HZ845 was designated as the second cluster, and the third cluster included HZ3, HZ5 and HZ Xia, and the fourth cluster included LD1, GY16, GY18, GY163, SM and XY-Xiu1. Meanwhile, the third cluster and the fourth cluster exhibited much closer phylogenetic relationship. The tested phylogenetic relationships among P. pulmonarius strains by ERIC-PCR were consistent with the results from antagonistic test and isozyme analysis of esterase. Therefore, ERIC-PCR is reliable in the identification of P. pulmonarius strains.

Key words: ERIC-PCR technique, antagonistic test, esterase isozyme, Pleurotus pulmonarius, phylogenetic relationship