High-performance Liquid Chromatography with Evaporative Light Scattering Detection for the Determination of Enzymatically Synthesized Lactosucrose

doi:10.7506/spkx1002-6630-201016038

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (16): 180-183.doi: 10.7506/spkx1002-6630-201016038

• Analysis & Detection • Previous Articles Next Articles

High-performance Liquid Chromatography with Evaporative Light Scattering Detection for the Determination of Enzymatically Synthesized Lactosucrose

Zheng RUAN

1.State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China ；
2.College of Life Science and Food Engineering, Nanchang University, Nanchang 330031, China；
3. Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China

Received:2009-11-24 Revised:2010-05-03 Online:2010-08-25 Published:2010-12-29
Contact: Zheng RUAN E-mail:ezruan@yahoo.com

Abstract

Abstract:

Objective: To develop a simple, accurate and sensitive high-performance liquid chromatographic (HPLC) method with evaporative light scattering detection (ELSD) for the determination of enzymatically prepared lactosucrose (LS) in our laboratory. Methods: The reaction product containing LS was centrifugated and the supernatant was diluted and separated on a Kromasil NH2 column using acetonitrile/water (75:25, V/V) mixture as mobile phase. The ELSD drift tube temperature was set at 70 ℃ and the air pressure at 0.35 MPa. Results: The linear range of LS was between 0.1281 mg/mL and 4.1000 mg/mL (R2 = 0.9994). The method detection limit was 0.01 mg/mL. The average spike recovery at 5 levels was 100.28%, with a relative standard deviation (RSD) of 3.07%. Conclusion: This method is simple, fast and accurate, and can be applied to the determination of LS.

Key words: high-performance liquid chromatography, evaporative light scattering detection, lactosucrose

CLC Number:

TS247

Zheng RUAN. High-performance Liquid Chromatography with Evaporative Light Scattering Detection for the Determination of Enzymatically Synthesized Lactosucrose[J]. FOOD SCIENCE, 2010, 31(16): 180-183.

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High-performance Liquid Chromatography with Evaporative Light Scattering Detection for the Determination of Enzymatically Synthesized Lactosucrose

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