Using Macroporous Resin for Enriching and Purifying Total Flavonoids from the Crude Extract of Eucommia ulmoides Oliv. Leaves

doi:10.7506/spkx1002-6630-201014015

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (14): 67-70.doi: 10.7506/spkx1002-6630-201014015

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Using Macroporous Resin for Enriching and Purifying Total Flavonoids from the Crude Extract of Eucommia ulmoides Oliv. Leaves

FU Gui-ming1，LUO Yang-fan2，ZHANG Ya-nan2，WAN Yin2，CHAI Jian-xin2，CHEN Yong-hui1，WU Xiao-fang1，CHEN Jian-fang1

1. Sino-German Food Engineering Centre, Nanchang University, Nanchang 330047, China；
2. College of Life Science and Food Engineering, Nanchang University, Nanchang 330047, China

Received:2009-11-12 Revised:2010-04-13 Online:2010-07-15 Published:2010-12-29
Contact: FU Gui-ming E-mail:wanyin_0@tom.cn

Abstract

Abstract:

In this study, the leaves of Eucommia ulmoides Oliv. were extracted with absolute ethanol to obtain flavonoid compounds. The crude extract was purified using 4 macroporous adsorption resins, and AB-8 was found to be the best choice due to both the highest adsorption and desorption capacity. The optimum conditions for adorption/desorption of the crude extract of the leaves of Eucommia ulmoides Oliv. were as follows: sample concentration 193.92 mg/mL at pH 2, sample flow rate 2.6 mL/min, desorption flow rate 1.6 mL/min and amount of 80% ethanol required for desorption 30 mL. After the optimized purification, the concentration of flavonoids was improved from 10.2% to 42.6%.

Key words: Eucommia ulmoides leaf flavonoids, enrichment and purification, macroporous resin

CLC Number:

FU Gui-ming1，LUO Yang-fan2，ZHANG Ya-nan2，WAN Yin2，CHAI Jian-xin2，CHEN Yong-hui1，WU Xiao-fang1，CHEN Jian-fang1. Using Macroporous Resin for Enriching and Purifying Total Flavonoids from the Crude Extract of Eucommia ulmoides Oliv. Leaves[J]. FOOD SCIENCE, 2010, 31(14): 67-70.

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Using Macroporous Resin for Enriching and Purifying Total Flavonoids from the Crude Extract of Eucommia ulmoides Oliv. Leaves

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