Abstract:

In this study, rapeseed meal protein prepared by reverse micelles extraction was sequentially hydrolyzed with two enzymes under ultrasonic assistance. Papain, protamex, alcalase, flavourzyme and trypsin were used to comprise 6 pairwise combinations for two-step hydrolysis of rapeseed meal protein. The hydrolysis efficacy of each combination was assessed using degree of hydrolysis (DH), trichloroacetic acid nitrogen-solution index (TCA-NSI), peptide yield and molecular weight distribution. The sequential hydrolysis with papain followed by flavourzyme or with trypsin followed by flavourzyme presented higher DH and peptide yield and the corresponding products both contained less small peptides.

Key words: rapeseed peptide, ultrasonic, stepwise enzymatic hydrolysis, enzyme screening