Abstract:

This paper reports a high-performance liquid chromatographic (HPLC) method based on matrix solid-phase dispersion followed by solid-phase extraction for the determination of melamine in complicated matrices such as animal feeds, chocolate products, meat products, and so on. The method developed had good immunity to the interferences of lipids and proteins and exhibited a lower false positive rate. Two calibration curves (detected at 215 or 240 nm) for melamine exhibited good linearity over the concentration range from 0.5 to 8.0μg/mL, with a correlation coefficient of more than 0.9995. The limit of detection of this method was 0.5 mg/kg.

Key words: matrix solid-phase dispersion, solid-phase extraction, complicated matrix, melamine