Microwave-assisted Enzymatic Hydrolysis of Corn Silk for Extraction of Polysaccharides

doi:10.7506/spkx1002-6630-201010009

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (10): 42-46.doi: 10.7506/spkx1002-6630-201010009

• Processing Technology • Previous Articles Next Articles

Microwave-assisted Enzymatic Hydrolysis of Corn Silk for Extraction of Polysaccharides

CHEN Hong，ZHANG Yan-rong，WANG Da-wei*，LIU Ting-ting，ZHANG Bo

College of Food Science and Engineering, Jilin Agricultural University, Changchun 130118, China

Received:2009-08-03 Revised:2009-12-01 Online:2010-05-15 Published:2010-12-29
Contact: WANG Da-wei E-mail:chenhong216@yahoo.com.cn

Abstract

Abstract:

The present study aimed at developing an optimal procedure for the extraction of polysaccharides from corn silk hydrolyzed with a commercial cellulase preparation under microwave assistance. The optimal values of crucial technological parameters for improved polysaccharides yield were determined by one-factor-at-a-time and orthogonal array design methods as follows: microwave powder 500 W for 2 min treatment, water/material ratio 30:1 (mL/g), enzyme dosage 1.5%, pH 5.0 and hydrolysis temperature 50 ℃ for a hydrolysis duration of 40 min. Under such conditions, a maximum polysaccharides yield of up to 8.12% was obtained.

Key words: corn silk, polysaccharides, extraction, microwave, cellulase

CLC Number:

TS242.9

CHEN Hong，ZHANG Yan-rong，WANG Da-wei*，LIU Ting-ting，ZHANG Bo. Microwave-assisted Enzymatic Hydrolysis of Corn Silk for Extraction of Polysaccharides[J]. FOOD SCIENCE, 2010, 31(10): 42-46.

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Microwave-assisted Enzymatic Hydrolysis of Corn Silk for Extraction of Polysaccharides

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