Abstract:

Used silica with different ligands was recovered and regenerated by means of acid treatment. Iminodiacetic acid (IDA)- recycled silica column was synthesized using IDA as new ligand and pickled silica with different ligands as matrix by direct or indirect methods. The chromatographic performance of synthesized packing materials was investigated. Results indicated that IDA-recycled silica column exhibited an excellent separation efficiency to proteins although its chromatographic performance was lower. IDA-recycled silica column synthesized by direct method revealed much better chromatographic performance than indirect synthesis method. The IDA-recycled silica column was successfully used to extract and purify lysozyme and cytochrome C from egg and pig heart, respectively. These investigations demonstrate the feasibility for recovery and regeneration of silica.

Key words: acid treatment, direct method, indirect method, IDA-recycled silica column, protein separation