Ultrasonic-assisted Acidic Acetone Extraction of Hemin from Porcine Blood

doi:10.7506/spkx1002-6630-201006024

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (6): 109-113.doi: 10.7506/spkx1002-6630-201006024

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Ultrasonic-assisted Acidic Acetone Extraction of Hemin from Porcine Blood

WANG Chang-guo1,2，DAI Ya1，LIU Shao-min2，YANG Wen-min1

1. Technical Research Center, Chuanyu Branch of China Tobacco Corporation, Chengdu 610066, China；
2. Department of Chemistry, University of Science and Technology of China, Hefei 230025, China

Received:2009-05-11 Revised:2009-10-23 Online:2010-03-15 Published:2010-12-29
Contact: WANG Chang-guo1 E-mail:wcgcx@163.com

Abstract

Abstract:

To modify the acidified acetone-sodium acetate extraction technique of hemin from porcine blood, ultrasonic was used to disrupt porcine red blood cells for accelerating the release of intracellular hemoglobin. Meanwhile, extracted hemin was structurally characterized by infrared spectroscopy and UV-Visible absorption spectroscopy, and quantitative analysis was carried out by UV-Visible absorption spectroscopy. Results showed that crucial parameters affecting ultrasonic disruption of porcine red blood cells such as ultrasonic power, water/ethanol volume ratio and ultrasonic treatment time were optimized to be 800 kW, 15:1 and 6 min, respectively. Under optimized parameters, 86.62% hemin with 92.67% purity was extracted. This method demonstrates the merits of simplicity, low cost and high purity of final products.

Key words: ultrasonic, acidified acetone method, hemin, extraction, orthogonal array design

CLC Number:

TS202.3

WANG Chang-guo1,2，DAI Ya1，LIU Shao-min2，YANG Wen-min1. Ultrasonic-assisted Acidic Acetone Extraction of Hemin from Porcine Blood[J]. FOOD SCIENCE, 2010, 31(6): 109-113.

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Ultrasonic-assisted Acidic Acetone Extraction of Hemin from Porcine Blood

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