宏基因组技术及其在酶制剂中的应用

doi:10.7506/spkx1002-6630-201309077

摘要/Abstract

摘要：

自然环境中有超过99%的微生物是不可培养的。使用宏基因组技术，人们首次实现了对不可培养微生物的开发，这为探索和挖掘新的酶制剂和活性小分子提供了更为有力的技术手段。本文重点介绍宏基因组技术最新的研究进展及其在新酶开发中的成功应用，以期利用宏基因组技术推进新酶开发，为其在食品工业中的应用提供有效途径。

关键词: 宏基因组, 宏基因文库, 酶制剂, 富集技术, DNA提取

Abstract:

More than 99% of microbes in natural environment are uncultivable. These uncultivable microorganisms have been
exploited for the first time by using metagenomic technology, especially as a more powerful technique for the exploration and
mining of novel enzymes and active small molecules. In this paper, the current research advances of metagenomic technology
and successful applications in the development of new enzymes have been summarized, which will accelerate the development
of new enzymes through metagenomic technology and provide an effective strategy for its application in the food industry.

Key words: metagenomic technology, macro-gene library, enzyme, enrichment technology, DNA extraction

中图分类号:

Q819

于雷，于丽，张薇，陆丽丽. 宏基因组技术及其在酶制剂中的应用[J]. 食品科学, 2013, 34(9): 404-407.

YU Lei，YU Li，ZHANG Wei，LU Li-li. Research Progress of Metagenomic Technology and Its Application in Enzyme Preparation[J]. FOOD SCIENCE, 2013, 34(9): 404-407.

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