• 安全检测 •

基于脂肪酸特征指标的鳕鱼肝油掺假鉴定

1. 1.舟山市食品药品检验检测研究院，浙江 舟山 316021；2.海力生集团有限公司，浙江 舟山 316021
• 出版日期:2019-04-25 发布日期:2019-05-05
• 基金资助:
浙江省食品药品监管系统科技计划项目（SP201627）

Adulteration Identification of Cod Liver Oils Based on Characteristic Fatty Acids

WANG Qiongfen, ZHENG Ping’an, LIU Ting, ZHANG Mengqi

1. 1. Zhoushan Institute for Food and Drug Control, Zhoushan 316021, China; 2. Hailisheng Group Co. Ltd., Zhoushan 316021, China
• Online:2019-04-25 Published:2019-05-05

Abstract: This study aimed to establish a method for identifying adulterated cod liver oils by analyzing the percentages of fatty acids in pure cod liver oil and the change of the characteristic fatty acids. The fatty acids in samples were methyl esterified by precolumn derivatization before being analyzed by gas chromatography (GC), and the percentage of each fatty acid in the total fatty acids was calculated by peak area normalization method. According to the results of multiplesample analysis, the ratio of docosahexaenoic acid (DHA) to eicosapentaenoic acid (EPA) and the content of cetoleic acid were identified as the characteristic indexes for authentication, which were in the range from 1.40 to 1.68 and from 7.0% to 9.4% in pure cod liver oil. Adulteration of fish oil in cod liver oil samples could be determined when the characteristic indexes exceeded the ranges. A quantitative model for the level of adulteration of cod liver oil was established based on these characteristic indexes, and discriminant analysis was performed for different levels of adulteration. Out of 46 batches of samples examined, 2 were suspected to be adulterated with fish oil at about 25%. This method is accurate, reliable, simple and feasible and can be used for the rapid identification of adulterated cod liver oil.