鱼油对KKAy糖尿病小鼠糖代谢及PI3K/Akt信号通路的影响

doi:10.7506/spkx1002-6630-201811020

食品科学 ›› 2018, Vol. 39 ›› Issue (11): 126-131.doi: 10.7506/spkx1002-6630-201811020

鱼油对KKAy糖尿病小鼠糖代谢及PI3K/Akt信号通路的影响

胡名媛1，王锋1，马永建1,2，孙桂菊1,*

1.东南大学公共卫生学院营养与食品卫生学系，环境医学工程教育部重点实验室，江苏南京 210009；2.江苏省疾病预防控制中心，江苏南京 210009

出版日期:2018-06-15 发布日期:2018-06-06
基金资助:
国家自然科学基金面上项目（81573144）

Effect of Fish Oil on Glucose Metabolism and PI3K/Akt Signaling Pathway in Diabetic KKAy Mice

HU Mingyuan1, WANG Feng1, MA Yongjian1,2, SUN Guiju1,*

1. Key Laboratory of Environmental Medicine and Engineering, Ministry of Education, Department of Nutrition and Food Hygiene, School of Public Health, Southeast University, Nanjing 210009, China; 2. Jiangsu Provincial Center for Disease Control and Prevention, Nanjing 210009, China

Online:2018-06-15 Published:2018-06-06

摘要/Abstract

摘要： 目的：探讨鱼油对KKAy糖尿病小鼠糖代谢及磷脂酰肌醇3-激酶（phosphatidylinositol 3-kinase，PI3K）/蛋白激酶B（protein kinase B，Akt）信号通路的影响。方法：40 只KKAy小鼠，分为模型组和鱼油低、中、高剂量组，每组10 只。另设10 只C57BL/6小鼠为正常对照组。灌胃12 周后，测定血糖、胰岛素、脂联素、瘦素及 PI3K/Akt信号通路中相关基因的表达水平。结果：与模型组相比，鱼油低剂量组血糖水平极显著降低（P＜0.01），鱼油低、中剂量组胰岛素水平显著提高（P＜0.05），鱼油各剂量组的脂联素水平显著提高（P＜0.05）。此外，鱼油各剂量组可上调胰岛素受体底物-1、PI3K、Akt、葡萄糖转运蛋白-4基因的表达，其中低剂量鱼油对Akt和葡萄糖转运蛋白-4基因的调控效果更显著。结论：低剂量鱼油可能通过调节PI3K/Akt信号通路改善糖代谢。

关键词: 2型糖尿病, 鱼油, 糖代谢, 胰岛素抵抗, PI3K/Akt信号通路

Abstract: Objective: To investigate the effect of fish oil on glucose metabolism and phosphatidylinositol 3-kinase (PI3K)/ protein kinase B (Akt) signaling pathway in diabetic KKAy mice. Methods: Forty KKAy mice were randomly assigned into four groups: diabetic model group, low-dose fish oil group, medium-dose fish oil group and high-dose fish oil group. Ten male C57BL/6 mice served as a non-diabetic control group. Blood and tissue samples were collected after 12 weeks of treatment. Blood glucose, serum insulin, adiponectin, leptin, and the mRNA expressions of insulin receptor substrate-1 (IRS-1), PI3K, Akt and glucose transporter-4 (GLUT-4) were detected. Results: Compared with the model group, fasting blood glucose was reduced significantly in the low-dose fish oil group (P < 0.01), fasting insulin was increased in both the low and medium-dose groups (P < 0.05), and adiponectin level was significantly increased in all the treatment groups (P < 0.05). In addition, fish oil at all doses up-regulated the mRNA expression of IRS-1, PI3K, Akt and GLUT-4 and at low dose had more significant effect on the mRNA expression of Akt and GLUT-4. Conclusion: Fish oil at low dose may improve glucose metabolism by regulating the PI3K/Akt signaling pathway.

Key words: type 2 diabetes mellitus, fish oil, glucose metabolism, insulin resistance, PI3K/Akt signaling pathway

中图分类号:

R151.4

胡名媛，王锋，马永建，孙桂菊. 鱼油对KKAy糖尿病小鼠糖代谢及PI3K/Akt信号通路的影响[J]. 食品科学, 2018, 39(11): 126-131.

HU Mingyuan, WANG Feng, MA Yongjian, SUN Guiju. Effect of Fish Oil on Glucose Metabolism and PI3K/Akt Signaling Pathway in Diabetic KKAy Mice[J]. FOOD SCIENCE, 2018, 39(11): 126-131.

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鱼油对KKAy糖尿病小鼠糖代谢及PI3K/Akt信号通路的影响

Effect of Fish Oil on Glucose Metabolism and PI3K/Akt Signaling Pathway in Diabetic KKAy Mice

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