食品科学 ›› 2021, Vol. 42 ›› Issue (17): 170-176.doi: 10.7506/spkx1002-6630-20200824-310

• 营养卫生 • 上一篇    下一篇

甜玉米芯多糖对胰岛素抵抗HepG2细胞糖代谢功能的影响

马永强,韩烨,张凯,王鑫,王峙力   

  1. (1.哈尔滨商业大学食品工程学院,黑龙江 哈尔滨 150076;2.哈尔滨工业大学生命科学与技术学院,黑龙江 哈尔滨 150080)
  • 发布日期:2021-09-29
  • 基金资助:
    哈尔滨商业大学青年创新人才项目(2019CX31);哈尔滨商业大学研究生创新基金项目(YJSCX2019-615HSD)

Effect of Sweet Corncob Polysaccharide on Glucose Metabolism in Insulin Resistant HepG2 Cells

MA Yongqiang, HAN Ye, ZHANG Kai, WANG Xin, WANG Zhili   

  1. (1. College of Food Engineering, Harbin University of Commerce, Harbin 150076, China; 2. School of Life Science and Technology, Harbin Institute of Technology, Harbin 150080, China)
  • Published:2021-09-29

摘要: 目的:探讨甜玉米芯多糖(sweet corncob polysaccharide,SCP)组分SCP-80-I对胰岛素抵抗HepG2(insulin resistant HepG2,IR-HepG2)细胞糖代谢功能的影响。方法:确立IR-HepG2细胞模型建立的最佳条件,并由此建立IR-HepG2细胞模型;分别以50、100、200、400 μg/mL剂量的SCP-80-I孵育细胞24 h,评价其对细胞葡萄糖消耗的影响,同时利用噻唑蓝法评价其细胞毒性;检测氧化应激标志物超氧化物歧化酶(superoxide dismutase,SOD)、丙二醛(malondialdehyde,MDA)及活性氧(reactive oxygen species,ROS)的水平,测定细胞内糖原积累水平及糖酵解关键限速酶己糖激酶(hexokinase,HK)、丙酮酸激酶(pyruvate kinase,PK)的活力。结果:确立以1×10-6 mol/L胰岛素处理24 h作为诱导IR-HepG2细胞形成的最佳条件,并成功建立IR-HepG2细胞模型;在孵育实验中,SCP-80-I能极显著增加IR-HepG2细胞的葡萄糖摄取量(P<0.01),细胞活力随SCP-80-I质量浓度的增加呈先上升后下降的趋势;200 μg/mL SCP-80-I处理组与模型对照组相比,胞内MDA含量极显著下降,SOD活力极显著提高,ROS水平极显著下降(P<0.01);胞内糖原含量极显著增加(P<0.01),HK与PK活力极显著增加(P<0.01)。结论:推测SCP-80-I的降血糖功效与其缓解氧化应激所造成的肝脏细胞损伤,改善胰岛素抵抗肝脏细胞的糖代谢功能有关。

关键词: 糖尿病;HepG2细胞;胰岛素抵抗;甜玉米芯多糖;糖代谢

Abstract: Objective: To investigate the effect of a polysaccharide isolated from sweet corncob (designated SCP-80-I) on glucose metabolism in insulin resistant (IR) HepG2 cells. Methods: The optimal conditions for inducing IR in HepG2 cells were established and applied. The IR cells were incubated for 24 h in the presence of different concentrations of SCP-80-I (50, 100, 200 and 400 μg/mL) to evaluate the effect of the polysaccharide on glucose uptake as well as assess the cytotoxicity by methyl thiazolyl tetrazolium (MTT) assay. Biomarkers of oxidative stress, including superoxide dismutase (SOD) activity, malondialdehyde (MDA) and reactive oxygen species (ROS) levels, were measured. In addition, intracellular glycogen accumulation and the activities of rate-limiting enzymes for glycolysis (hexokinase, HK; pyruvate kinase, PK) were determined. Results: Treatment with 1 × 10-6 mol/L insulin for 24 hours was the optimal condition for the formation of IR HepG2 cells. SCP-80-I significantly increased glucose uptake in IR HepG2 cells (P < 0.01), and the cell viability increased first and then decreased with increasing SCP-80-I concentration. The contents of MDA and ROS decreased, and the activities of SOD, HK and PK and intracellular glycogen contents increased in the SCP-80-I intervention group (200 μg/mL) compared with the model control group (P < 0.01). Conclusion: The hypoglycemic effect of SCP-80-I may be related to the alleviation of oxidative stress-induced liver injury and the improvement of glucose metabolism in IR liver cells.

Key words: diabetes mellitus; HepG2 cells; insulin resistance; sweet corncob polysaccharide; glucose metabolism

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