• 安全检测 •

### 高效液相色谱法检测活性食品包装薄膜中的香芹酚和百里香酚

1. （上海海洋大学食品学院，上海 201306）
• 出版日期:2018-08-25 发布日期:2018-08-17
• 基金资助:
上海市教育委员会科研创新项目（14YZ120）；国家自然科学基金面上项目（31571866）

### High Performance Liquid Chromatography Method for Rapid Detection of Carvacrol and Thymol in Active Food Packaging Film

YANG Chunxiang, WANG Yifen, WANG Jing*, LI Li, WEN Yihua, YANG Binghao

1. (College of Food Sciences and Technology, Shanghai Ocean University, Shanghai 201306, China)
• Online:2018-08-25 Published:2018-08-17

Abstract: A method was developed for determining carvacrol and thymol in modified polylactic acid (PLA) active food packaging film by high performance liquid chromatography (HPLC). Samples were analyzed by HPLC on a Sunfire C18 column with 10 μL of a mobile phase consisting of acetonitrile and water (3:2, V/V) at a flow rate of 1 mL/min by gradient elution. The column temperature was set at (30 ± 5) ℃ and the analysis time was 10 min. The detection wavelength was 274 nm and the external standard method was used for quantitative analysis. Good linearities for the analytes were obtained in the concentration range of 5–100 mg/L with correlation coefficients of more than 0.996. The recoveries from samples spiked with carvacrol and thymol were 71.6%–93.7% and 73.1%–103.6%, with relative standard deviations (RSDs) of 5.2%–8.3% and 5.0%–8.8%, respectively. The limits of detection (LODs) for the analytes were 200 μg/kg and limits of quantification (LOQs) were 500 μg/kg. The method proved to be rapid, simple and highly sensitive, and it could provide a foundation for gaining further insight into the release mechanism of carvacrol and thymo from active food packaging materials and ensuring the safe application of active food packaging film.