• 工艺技术 •

### 双水相法制备鳜鱼（Siniperca chuatsi）肌动蛋白及定量分析

1. （1.暨南大学生命科学技术学院，广东 广州 510632；2.暨南大学理工学院，广东 广州 510632）
• 出版日期:2020-06-25 发布日期:2020-06-22
• 基金资助:
“十三五”国家重点研发计划重点专项（2016YFD0401203）

### Preparation of Actin from Siniperca chuatsi Muscle Using Aqueous Two-Phase System and Its Quantitative Analysis

WANG Yangyang, XU Mingfang, BAI Weibin, ZHENG Chunli, YE Lei, ZHENG Qinqin

1. (1. College of Life Science and Technology, Jinan University, Guangzhou 510632, China; 2. College of Science and Engineering, Jinan University, Guangzhou 510632, China)
• Online:2020-06-25 Published:2020-06-22

Abstract: In this study, a method for the extraction and purification of actin from Siniperca chuatsi myofibrillar protein by alcohol-inorganic salt aqueous two-phase systems was established. A new method for the quantitative detection of S. chuatsi actin was explored using high performance micellar electrokinetic capillary chromatography (MECC) with diode array detection (DAD). The results showed that an isopropanol/ammonium sulfate aqueous two-phase system was determined as the optimal separation system by actin purity detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and extraction efficiency. Using combination of one-factor-at-a-time method and response surface methodology, the optimal aqueous two-phase system was determined to be composed of 26% isopropanol and 11% ammonium sulfate at pH 9.0, giving an actin extraction yield of 11.04%, which was close to the predicted value of the model. The actin peak occurred at around 10 minutes in the capillary electrophoresis spectrum, whose baseline was smooth without any noise peak. The results from this study lay an experimental foundation for the identification of quality attributes of precious fish and for development of fish actin standard products, and also provide a theoretical basis for the development and utilization of fish protein functions and rapidquantitative detection of fish proteins.