食品科学 ›› 2007, Vol. 28 ›› Issue (3): 228-233.

• 生物工程 • 上一篇    下一篇

源于不同菌株的谷氨酰胺转胺酶对酪蛋白的作用研究

 马微, 王海波, 付丽, 李春, 张兰威   

  1. 东宁出入境检验检疫局; 郑州牧业工程高等学校; 东北农业大学; 哈尔滨工业大学 黑龙江东宁157200; 黑龙江东宁157200; 河南郑州450011; 黑龙江哈尔滨150030;
  • 出版日期:2007-03-15 发布日期:2011-12-31

Catalysis Study on Casein Transglutaminase from Different Strains

 MA  Wei, WANG  Hai-Bo, FU  Li, LI  Chun, ZHANG  Lan-Wei   

  1. 1.Dongning Entry-Exit Inspection and Quarantine Bureau, Dongning 157200, China; 2.Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China; 3.Northeast Agricultural University, Harbin 150030, China; 4.Harbin Institute of Technology, Harbin 150030, China
  • Online:2007-03-15 Published:2011-12-31

摘要: 本实验采用SDS-PAGE电泳和凝胶成像技术对TG-K和TG-N与酪蛋白反应的最佳条件(酶用量、反应时间、反应温度、反应pH值)和作用效果进行了对比分析,从而进一步探讨了源于不同菌株的谷氨酰胺转胺酶对酪蛋白的作用和酶本身的特性研究,为该酶在乳制品加工中的应用提供了理论指导。

关键词: 谷氨酰胺转胺酶, 酪蛋白, 不同菌种, 聚合物

Abstract: The appropriate conditions(enzyme/usage, time, temperature and pH ) for catalytic reactions and the effects on casein, using TG-K and TG-N were compared and analyzed by SDS-PAGE combined with the technique of imaging. Accordingly, this paper ulterior research catalysis study on casein by transglutaminase from different strain and the enzyme characters, and provide direction for application of transglutaminase in diary processing.

Key words: transglutaminase, casein, different strain, biopolymer