食品科学 ›› 2021, Vol. 42 ›› Issue (4): 221-226.doi: 10.7506/spkx1002-6630-20200527-324

• 成分分析 • 上一篇    下一篇

核磁共振磷谱法测定乳制品中的酪蛋白含量

冯翠萍,刘一诺,樊双喜,武竹英,钟其顶   

  1. (1.中国食品发酵工业研究院有限公司,北京 100015;2.全国食品发酵标准化中心,北京 100015;3.国家食品质量监督检验中心,北京 100015)
  • 出版日期:2021-02-25 发布日期:2021-02-25
  • 基金资助:
    科技部国家重点研发计划项目(2016YFE0113200;2017YFE0110800); 国家自然科学基金青年科学基金项目(31601553;31601580);科技部“十三五”重大科技计划项目(2016YFF0203903); 欧盟地平线2020计划项目(635690-OLEUM;727864-EU-China-Safe)

Determination of Casein Content in Dairy Products by 31P Nuclear Magnetic Resonance

FENG Cuiping, LIU Yinuo, FAN Shuangxi, WU Zhuying, ZHONG Qiding   

  1. (1. China National Institute of Food and Fermentation Industries, Beijing 100015, China; 2. National Standardization Center of Food and Fermentation Industry, Beijing 100015, China; 3. National Food Quality Supervision and Inspection Center, Beijing 100015, China)
  • Online:2021-02-25 Published:2021-02-25

摘要: 基于插值法建立乳制品中酪蛋白的核磁共振磷谱定量检测方法。结果表明,该方法的检出限为0.38 g/L(信噪比(RSN)=3),定量限为1.25 g/L(RSN=10);在5.00~35.00 g/L质量浓度范围内线性良好,相关系数R2大于0.999;加标回收率在91.94%~105.10%范围区间;日内精密度在0.65%~1.40%范围区间;日间精密度在1.40%~1.80%范围区间。对市售不同乳制品中酪蛋白含量进行检测,该方法与GB 31638—2016《酪蛋白》测定结果误差在±5%以内,满足方法可行性对比分析验证要求。该方法相比常规方法样品前处理简单、定量准确性高,大大缩短了检测时间,且有更广泛的适用性,满足乳制品中酪蛋白快速定量检测的要求。

关键词: 核磁共振磷谱;插值法;定量;酪蛋白;乳制品

Abstract: In this study, we established a quantitative method for the detection of casein in dairy products using 31P nuclear magnetic resonance (NMR) combined with the interpolation method. The results showed that the limit of detection (LOD) of this method was 0.38 g/L (RSN = 3), and the limit of quantitation (LOQ) was 1.25 g/L (RSN = 10). Good linearity was observed within the concentration range of 5.00–35.00 g/L, with correlation coefficient R2 greater than 0.999. The spiked recoveries were in the range of 91.94%–105.10%. The intra-day and inter-day precisions (relative standard deviation, RSD) were in the range of 0.65%–1.40% and 1.40%–1.80%, respectively. The casein contents of different dairy products on the market were detected by this method and the current national standard method (GB 31638-?2016). The error between the two methods was within ± 5%, which met the requirements for comparative analysis and validation of feasibility. Compared with the conventional method, this method had the advantages of simpler sample pretreatment, higher quantitative accuracy, greatly shorter detection time and wider applicability. Therefore, it could be suitable for rapid determination of casein content in dairy products.

Key words: 31P nuclear magnetic resonance; interpolation method; quantitation; casein; dairy products

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