次氯酸钠对德尔卑沙门氏菌生物被膜的抑制作用及机制

doi:10.7506/spkx1002-6630-20200615-201

食品科学 ›› 2021, Vol. 42 ›› Issue (13): 1-9.doi: 10.7506/spkx1002-6630-20200615-201

• 基础研究 • 下一篇

次氯酸钠对德尔卑沙门氏菌生物被膜的抑制作用及机制

闫玉卿，张一敏，董鹏程，毛衍伟，梁荣蓉，朱立贤，罗欣

（1.山东农业大学食品科学与工程学院，山东泰安 271018；2.江苏省肉类生产与加工质量安全控制协同创新中心，江苏南京 210095）

出版日期:2021-07-15 发布日期:2021-07-27
基金资助:
“十三五”国家重点研发计划项目（2019YFE0103800）；山东省现代农业产业技术体系牛产业创新团队项目（SDAIT-09-09）；现代农业产业技术体系建设专项（CARS-037）；山东省“双一流”奖补资金项目（SYL2017XTTD12）

Biofilm Inhibition Activity and Mechanism of Action of Sodium Hypochlorite against Salmonella Derby

YAN Yuqing, ZHANG Yimin, DONG Pengcheng, MAO Yanwei, LIANG Rongrong, ZHU Lixian, LUO Xin

(1. College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, China; 2. Jiangsu Collaborative Innovation Center of Meat Production and Processing, Quality and Safety Control, Nanjing 210095, China)

Online:2021-07-15 Published:2021-07-27

摘要/Abstract

摘要： 目的：为控制德尔卑沙门氏菌（Salmonella Derby）在食品加工过程中的污染，研究不同体积分数次氯酸钠对S. Derby生物被膜的抑制作用及其机制。方法：采用结晶紫染色法和平板菌落计数法观察次氯酸钠对生物被膜的抑制和清除效果，进一步观察不同体积分数次氯酸钠作用下沙门氏菌泳动能力和生物被膜内细胞的代谢活性、胞外聚合物含量和微观形态的变化规律。结果：次氯酸钠的最小抑菌浓度（minimum inhibitory concentration，MIC）为0.08%（体积分数，下同），亚MIC（0.04%、0.02%、0.01%）的次氯酸钠可以抑制S. Derby生物被膜的形成，对成熟生物被膜也有一定的清除效果，且不同亚MIC次氯酸钠的作用效果差异显著（P＜0.05）；不同体积分数次氯酸钠处理能降低生物被膜内细胞代谢活性，抑制胞外多糖的合成，且不同体积分数次氯酸钠的作用效果差异显著（P＜0.05）。激光共聚焦扫描显微镜结果显示，与对照组相比，次氯酸钠处理使得生物被膜厚度减小、活菌数减少、死菌数增多、细菌由聚集状态变为分散状态。结论：不同体积分数次氯酸钠对S. Derby生物被膜的形成有明显的抑制作用，这一作用与干扰细菌生长、生物被膜内细胞代谢活性和胞外多糖的合成有关。

关键词: 德尔卑沙门氏菌；生物被膜；次氯酸钠；代谢活性；胞外聚合物

Abstract: Purpose: In order to control the contamination of Salmonella Derby during food processing, the inhibitory effect of different concentrations of sodium hypochlorite on the biofilm formation of Salmonella Derby was studied and the underlying mechanism was investigated. Methods: The crystal violet staining and plate counting methods were used to observe the potential of sodium hypochlorite to inhibit and eradicate Salmonella Derby biofilm, and the content of extracellular polymeric substances (EPS), biofilm metabolism, micromorphology and swimming mobility of Salmonella Derby in the presence of sodium hypochlorite at different concentrations were observed. Results: The minimum inhibitory concentration (MIC) of sodium hypochlorite was 0.08% (V/V), and the sub-MICs (0.04%, 0.02% and 0.01%) of sodium hypochlorite could inhibit the formation of Salmonella Derby biofilm, and also eradicate mature biofilms and the effect of sodium hypochlorite with different sub-MICs was significantly different (P < 0.05). Sodium hypochlorite could significantly reduce biofilm metabolism of Salmonella Derby and inhibit biofilm exopolysaccharide synthesis, and the effect of sodium hypochlorite with different concentrations was significantly different (P < 0.05). The results of confocal laser scanning microscopy (CLSM) showed that sodium hypochlorite treatment greatly reduced the thickness of the biofilm, reduced the number of live bacterial cells, increased the number of dead bacterial cells, and transform the biofilm cells from an aggregated state to a dispersed one. Conclusion: Sodium hypochlorite at different sub-MICs has a significant inhibitory effect on the formation of Salmonella Derby biofilms, possibly by interfering with bacterial growth, metabolism and extracellular polysaccharide secretion.

Key words: Salmonella Derby; biofilm; sodium hypochlorite; metabolism; extracellular polymeric substance

中图分类号:

TS201.3

闫玉卿，张一敏，董鹏程，毛衍伟，梁荣蓉，朱立贤，罗欣. 次氯酸钠对德尔卑沙门氏菌生物被膜的抑制作用及机制[J]. 食品科学, 2021, 42(13): 1-9.

YAN Yuqing, ZHANG Yimin, DONG Pengcheng, MAO Yanwei, LIANG Rongrong, ZHU Lixian, LUO Xin. Biofilm Inhibition Activity and Mechanism of Action of Sodium Hypochlorite against Salmonella Derby[J]. FOOD SCIENCE, 2021, 42(13): 1-9.

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次氯酸钠对德尔卑沙门氏菌生物被膜的抑制作用及机制

Biofilm Inhibition Activity and Mechanism of Action of Sodium Hypochlorite against Salmonella Derby

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