食品科学 ›› 2009, Vol. 30 ›› Issue (24 ): 412-415.doi: 10.7506/spkx1002-6630-200924094

• 分析检测 • 上一篇    下一篇

高效毛细管区带电泳法测定鸡肉中肌苷酸含量

周梅仙,周业飞   

  1. 南京晓庄学院生命科学系
  • 收稿日期:2009-07-22 出版日期:2009-12-15 发布日期:2010-12-29
  • 通讯作者: 周梅仙, E-mail:zhoumeixian6890@126.com
  • 基金资助:

    南京晓庄学院院级重点项目(2008NXY10)

Determination of IMP in Chicken by Capillary Zone Electrophoresis

ZHOU Mei-xian,ZHOU Ye-fei   

  1. ZHOU Mei-xian,ZHOU Ye-fei
  • Received:2009-07-22 Online:2009-12-15 Published:2010-12-29
  • Contact: ZHOU Mei-xian, E-mail:zhoumeixian6890@126.com

摘要:

建立了毛细管区带电泳法测定鸡肉中肌苷酸含量的分析方法。采用20mmol/L 硼酸缓冲液(pH9.3)作为缓冲体系,在20℃、30kV 和检测波长为254nm 的条件下测定。结果显示,肌苷酸质量浓度在1.7~30μg/ml 范围内与相应的峰面积呈良好的线性关系(r=0.9983),方法重现性好,迁移时间和峰面积RSD 分别为0.27% 和2.74%(n=5)。该法快速、灵敏度高,为测定鸡肉中肌苷酸含量提供了一种高效、快速、简便的方法。

关键词: 毛细管区带电泳, 鸡肉, 肌苷酸

Abstract:

An easy and low-cost method for determining inosine monophosphate (IMP) in chicken by capillary zone electrophoresis (CZE) was established. The capillary zone electrophoresis was conducted in the buffer of 20 mmol/L borate (pH 9.3) at the voltage of 30 kV and detection wavelength of 254 nm. Results indicated that an excellent linear relationship between IMP concentration and peak area was observed (r = 0.9983). The relative standard deviations for migration time and peak area were less than 0.27% and 2.74%, respectively. This capillary electrophoresis method provided simple, rapid, convenient and high reproducible determination, which was suitable for the determination of IMP in chicken.

Key words: capillary zone electrophoresis, chicken, IMP

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