食品科学 ›› 2021, Vol. 42 ›› Issue (16): 293-303.doi: 10.7506/spkx1002-6630-20200827-377

• 安全检测 • 上一篇    下一篇

基于液相色谱-四极杆飞行时间质谱方法分析冰鲜鸡肉代谢标志物

王珂雯,徐雷,徐贞贞,王雪,杨曙明   

  1. (1.中国农业科学院农业质量标准与检测技术研究所,农业农村部农产品质量安全重点实验室,北京 100081;2.中国农业大学食品科学与营养工程学院,北京 100083)
  • 发布日期:2021-08-27
  • 基金资助:
    2018年国家畜禽产品质量安全风险评估项目(GJFP201800704)

Analysis of Metabolic Markers in Chilled Chicken Based on Liquid Chromatography-Quadruple Time-of-Flight Mass Spectrometry

WANG Kewen, XU Lei, XU Zhenzhen, WANG Xue, YANG Shuming   

  1. (1. Key Laboratory of Agro-food Safety and Quality, Ministry of Agriculture and Rural Affairs, Institute of Quality Standard & Testing Technology for Agro-products, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2. College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China)
  • Published:2021-08-27

摘要: 采用液相色谱-四极杆飞行时间质谱(liquid chromatography-quadruple time-of-flight mass spectrometry,LC-QTOF MS)结合正交偏最小二乘(orthogonal partial least square,OPLS)法分析不同贮存时间的冰鲜鸡肉(1、3、5、7、10?d)和鲜鸡肉(0?d)的代谢物,从中筛选出29?个差异代谢物,主要包括蛋白质、脂肪和核苷酸降解产物。在冰鲜鸡肉贮存过程中,苯丙氨酸、色氨酸、棕榈酸和二十碳五烯酸含量显著上升,肌肽、鹅肌肽和肌苷酸等物质含量显著降低,并且发现贮存5?d以内的冰鲜鸡肉和鲜鸡肉在代谢物组成上具有更多的相似性。本实验表明,基于LC-QTOF?MS技术的代谢组学方法结合OPLS模型对冰鲜鸡肉和鲜鸡肉的鉴别具有可行性,本研究为冰鲜鸡肉代谢物研究提供思路,为冰鲜鸡肉的质量控制及品质评价提供数据支撑。

关键词: 冰鲜鸡肉;鲜鸡肉;贮存时间;代谢物;正交偏最小二乘法

Abstract: Liquid chromatography-quadruple time-of-flight mass spectrometry (LC-QTOF-MS) combined with orthogonal partial least square (OPLS) was used for metabolite analysis in chilled chicken (stored for 1, 3, 5, 7 and 10 days) and fresh chicken. Twenty-nine differential compounds including amino acids, peptides, fatty acids and nucleotides were obtained in the experiment. With increasing storage period, the contents of phenylalanine, tryptophan, palmitic acid and eicosatetraenoic acid were significantly increased, while the contents of L-carnosine, L-anserine and 5’-IMP were significantly decreased. Moreover, chilled chicken stored for less than 5 days had more similarity with fresh chicken in metabolite composition. This study indicates that it is feasible to discriminate chilled from fresh chicken using metabolomics based on LC-QTOF-MS combined with OPLS model, which provides a novel idea for exploring metabolites in chilled chicken, and provides data support for the quality control and evaluation of chilled chicken.

Key words: chilled chicken; fresh chicken; storage time; metabolites; orthogonal partial least square

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