家禽定点屠宰场不同屠宰区域空气的微生物结构

doi:10.7506/spkx1002-6630-201821033

食品科学 ›› 2018, Vol. 39 ›› Issue (21): 219-223.doi: 10.7506/spkx1002-6630-201821033

家禽定点屠宰场不同屠宰区域空气的微生物结构

戴宝玲1,2，肖英平2，孙凤来3，王佩佩1,2，桂国弘2，戴贤君1，杨华2,*

1.中国计量大学生命科学学院，浙江杭州 310018；2.浙江农业科学院农产品质量标准研究所，浙江杭州 310021；3.杭州余杭区仁和街道农业公共服务中心，浙江杭州 310000

出版日期:2018-11-15 发布日期:2018-11-21
基金资助:
杭州市科技局社会发展项目（20150533B77）；浙江省植物有害生物防控重点实验室——省部共建国家重点实验室培育基地项目（2010DS700124-ZM1608）

Structure of Airborne Microbial Communities in Different Slaughter Areas of Poultry Slaughterhouse

DAI Baoling1,2, XIAO Yingping2, SUN Fenglai3, WANG Peipei1,2, GUI Guohong2, DAI Xianjun1, YANG Hua2,*

1. College of Life Science, China Jiliang University, Hangzhou 310018, China; 2. Institute of Quality and Standard for Agro-products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; 3. Renhe District Hangzhou Yuhang Agricultural Public Service Center, Hangzhou 310000, China

Online:2018-11-15 Published:2018-11-21

摘要/Abstract

摘要： 为了解家禽屠宰场空气中微生物分布情况及其结构特点，在浙江选择一家禽定点屠宰场，以菌落总数为指标，比较分析屠宰前和屠宰后各屠宰区域空气沉降微生物的数量，并提取微生物DNA作为模板，经聚合酶链式反应扩增细菌16S rRNA基因的V3～V4区，利用高通量测序技术分析其菌群结构。结果发现家禽屠宰场的不同屠宰区域空气沉降微生物数量有所差异，同时屠宰后微生物数量比屠宰前有所增加，特别是在屠宰后挂禽间菌落总数比屠宰前高出71%。高通量测序显示屠宰前和屠宰后不同屠宰区域空气沉降菌多样性、菌群结构、相对丰度等方面均无显著性差异（P＞0.05），主要分布于6 个门，优势菌门为厚壁菌门（Firmicutes）、变形菌门（Proteobacteria）和放线菌门（Actinobacteria），总的相对丰度在84%以上。在属的水平上，葡萄球菌属（Staphylococcus）和不动细菌属（Acinetobacter）为优势菌属，分别占36.90%和13%（在前10 个属中），此外，在前35 个属中，葡萄球菌属（Staphylococcus）、寡养单胞菌属（Stenotrophomonas）和埃希菌属（Escherichia）等条件致病菌属分别占33.75%、4.65%和0.90%。由此说明屠宰场不同屠宰区域沉降菌数量存在一定差异，各屠宰区域均分布有一定数量的条件致病菌，研究为鸡肉微生物污染的溯源和完善家禽屠宰场卫生管理提供科学依据。

关键词: 鸡肉, 空气微生物, 高通量测序, 菌群结构

Abstract: In order to understand the distribution and structure of airborne microbial communities in poultry slaughterhouses, the quantity of airborne microorganisms in different areas of a slaughterhouse in Zhejiang province before and after slaughter was compared by measuring the total number of colonies, and total bacterial genomic DNA was extracted from the air samples and used as a template to amplify and sequence the V3–V4 region of the 16S rRNA gene by high throughout sequencing. The results showed that there was a difference in the number of airborne microorganisms in different slaughter areas of the poultry slaughterhouse and the microbial quantity after slaughter was higher than before slaughter. Especially, the total number of colonies after slaughter was 71% higher than before slaughter in the poultry hanging area. High throughput sequencing showed no significant differences in the diversity, structure and abundance of bacterial communities before and after slaughter for all slaughter areas (P > 0.05). The majority of the identified bacteria belonged to 6 phyla, and the dominant bacteria were Firmicutes, Proteobacteria and Actinobacteria, with total relative abundance of more than 84%. In the top 10 genera, Staphylococcus and Acinetobacter were the dominant bacteria, accounting for 36.90% and 13%, respectively. In addition, Staphylococcus, Stenotrophomonas and Escherichia as conditional pathogenic bacteria accounted for 33.75%, 4.65% and 0.90% in the top 35 genera, respectively. These data suggested that there were some differences in the number of airborne bacteria in different slaughter areas, and a certain number of conditional pathogens were detected in air samples from each area. In conclusion, this study provides a scientific basis for the traceability of microbial contaminants in chicken and improving poultry slaughterhouse hygiene management.

Key words: chicken, airborne microorganisms, high throughput sequencing, microbial flora structure

中图分类号:

S831.4＋5

戴宝玲，肖英平，孙凤来，王佩佩，桂国弘，戴贤君，杨华. 家禽定点屠宰场不同屠宰区域空气的微生物结构[J]. 食品科学, 2018, 39(21): 219-223.

DAI Baoling, XIAO Yingping, SUN Fenglai, WANG Peipei, GUI Guohong, DAI Xianjun, YANG Hua. Structure of Airborne Microbial Communities in Different Slaughter Areas of Poultry Slaughterhouse[J]. FOOD SCIENCE, 2018, 39(21): 219-223.

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家禽定点屠宰场不同屠宰区域空气的微生物结构

Structure of Airborne Microbial Communities in Different Slaughter Areas of Poultry Slaughterhouse

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