稻花香活力型白酒对大鼠TLR4在蛋白及基因水平表达的影响

食品科学 ›› 2008, Vol. 29 ›› Issue (7): 438-441.

稻花香活力型白酒对大鼠TLR4在蛋白及基因水平表达的影响

侯彩云,陈超,刘朝奇

三峡大学医学院药理学教研室；三峡大学医学院分子生物学研究所湖北宜昌443002；湖北宜昌443002

出版日期:2008-07-15 发布日期:2011-07-28

Effects of Daohuaxiang Vigor Liquor on TLR4 Expression on Protein and Gene Levels of Rats

HOU Cai-Yun, CHEN Chao, LIU Chao-Qi

1.Department of Pharmacology, College of Medical, China Three Gorges University, Yichang 443002, China; 2.Research Institute of Molecular Biology, College of Medical, China Three Gorges University, Yichang 443002, China

Online:2008-07-15 Published:2011-07-28

摘要/Abstract

摘要： 目的:同步检测TLR4在蛋白及基因水平的表达,观察稻花香活力型白酒(DVL)和普通稻花香白酒(DCL)对大鼠肝组织TLR4表达的影响,以比较这两种酒毒性的差异,并探讨白酒毒性及肝损伤的机理。方法:将28只SD大鼠随机分为DVL组、DCL组和正常对照组,采用大剂量白酒灌胃法制备大鼠急性酒精性肝损伤模型,应用免疫组化和RT-PCR法检测肝组织中TLR4的蛋白及基因表达情况。结果:免疫组化和RT-PCR检测结果均显示,DCL组大鼠肝组织中TLR4的表达量显著升高,而DVL组TLR4的表达量显著低于DCL组。结论:与普通稻花香白酒相比,稻花香活力型白酒可显著下调肝组织中TLR4的表达,从而减轻了对肝脏的损害。

关键词: 稻花香活力型白酒(DVL), TLR4, 肝损伤

Abstract: Objective: To detect TLR4 expression on protein and gene levels synchronously, investigate the effects of DVL (Daohuaxiang vigor liquor) and DCL (Daohuaxiang common liquor) on TLR4 expression in hepatic tissue of rat for differentiat-ing of differences of their toxicity, and probe into the mechanism of toxicity and liver injury induced by alcohol. Methods: 28 SD rats were randomly divided into DVL group, DCL group and normal control group. The rat model of acute liver injury was established by administration with high dose of above liquors. The expression of TLR4 on protein and gene levels in hepatic tissue was measured by immunohistochemistry and real-time RT-PCR methods. Results: The results of immunohistochemistry and RT-PCR both demonstrated that TLR4 expression of hepatic tissue is obviously increased in DCL group. However, the expression amount of TLR4 in DVL group is significantly lower than that in DCL group. Conclusion:DVL can obviously decline the TLR4 expression level in hepatic tissue so as to relieve the liver injury compared with DCL.

Key words: Daohuaxiang vigor liquor (DVL), TLR4 (Toll-like receptor 4), liver injury

侯彩云, 陈超, 刘朝奇. 稻花香活力型白酒对大鼠TLR4在蛋白及基因水平表达的影响[J]. 食品科学, 2008, 29(7): 438-441.

HOU Cai-Yun, CHEN Chao, LIU Chao-Qi. Effects of Daohuaxiang Vigor Liquor on TLR4 Expression on Protein and Gene Levels of Rats[J]. FOOD SCIENCE, 2008, 29(7): 438-441.

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