食品科学 ›› 2020, Vol. 41 ›› Issue (7): 159-163.doi: 10.7506/spkx1002-6630-20190321-285

• 营养卫生 • 上一篇    下一篇

小麦低聚肽对急性酒精中毒小鼠抗氧化功能的影响

于兰兰,刘伟,周雅琳,李雍,秦勇,李睿珺,许雅君   

  1. (北京大学公共卫生学院营养与食品卫生学系,食品安全毒理学研究与评价北京市重点实验室,北京 100191)
  • 出版日期:2020-04-15 发布日期:2020-04-20
  • 基金资助:
    新疆维吾尔自治区重点研发计划项目(2017B03020-2)

Effects of Wheat Oligopeptides on Antioxidant Function of Mice with Acute Alcoholism

YU Lanlan, LIU Wei, ZHOU Yalin, LI Yong, QIN Yong, LI Ruijun, XU Yajun   

  1. (Beijing Key Laboratory Toxicological Research and Risk Assessment for Food Safety, Department of Nutrition and Food Hygiene, School of Public Health, Peking University, Beijing 100191, China)
  • Online:2020-04-15 Published:2020-04-20

摘要: 通过研究小麦低聚肽对乙醇氧化损伤小鼠血清和肝脏组织中氧化损伤及抗氧化指标的影响,初步评价小麦低聚肽对急性酒精中毒小鼠是否具有抗氧化功能。将60 只清洁级健康雄性ICR小鼠按体质量随机分为空白对照组、模型组及小麦低聚肽低、中、高剂量组(0.4、0.8、1.6 g/kg mb),连续灌胃30 d,末次灌胃后,模型组和小麦低聚肽3 个剂量组小鼠禁食26 h,再以12 mL/kg mb剂量灌胃体积分数50%乙醇溶液,空白组不作处理;6 h后摘眼球取血处死小鼠,测定小鼠血清、肝脏中总超氧化物歧化酶(total superoxide dismutase,T-SOD)活力、谷胱甘肽(glutathione,GSH)水平、丙二醛(malondialdehyde,MDA)水平及肝脏组织中蛋白质羰基含量。结果表明:与模型组相比,小麦低聚肽各剂量组小鼠肝脏中T-SOD活力均极显著提高(P<0.01);小麦低聚肽低剂量组小鼠血清(P<0.05)和肝脏(P<0.01)中GSH水平显著升高、肝脏中蛋白质羰基含量显著降低(P<0.05);小麦低聚肽高剂量组小鼠血清中MDA浓度显著降低(P<0.05)。提示小麦低聚肽能提高急性酒精中毒小鼠体内抗氧化能力,对小鼠急性酒精性肝损伤具有一定的保护作用。

关键词: 小麦低聚肽, 急性酒精中毒, 酒精性肝损伤, 氧化损伤, 抗氧化

Abstract: The effects of wheat oligopeptides on ethanol-induced oxidative damage and antioxidant indexes in serum and liver tissues of mice were evaluated to preliminarily determine whether wheat oligopeptides can improve the antioxidant status of mice with acute alcoholism. Sixty healthy male ICR mice were randomly divided into five groups by body mass: blank control group, model group, and low-, medium- and high-dose wheat oligopeptides intervention groups (0.4, 0.8 and 1.6 g/kg mb), and all these animals were administered by gavage for 30 days. After the last gavage, the mice in the model group and the three wheat oligopeptides intervention groups were fasted for 26 h and then re-gavaged with 50% ethanol aqueous solution at a dose of 12 mL/kg mb, while those in the blank group were not treated. The activity of total superoxide dismutase (T-SOD), and the levels of glutathione (GSH), malondialdehyde (MDA) and protein carbonyl (PC) in the serum and liver of all mice were determined after 6 hours. The results showed that compared with the model control group, the activity of T-SOD in liver was significantly increased in each intervention group (P < 0.01). In the low-dose wheat oligopeptide group, the contents of GSH in serum (P < 0.05) and liver (P < 0.01) were both significantly increased, and the content of PC in liver was significantly decreased (P < 0.05). Wheat oligopeptides at high dose could significantly reduce the content of MDA in serum (P < 0.05). These results suggest that wheat oligopeptides can enhance the antioxidant capacity in mice with acute alcoholism and have a protective effect on acute alcoholic liver injury in mice.

Key words: wheat oligopeptides, acute alcoholism, alcoholic liver injury, oxidative damage, antioxidant

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