灵芝肽对小鼠半乳糖胺致肝损伤的保护作用

食品科学 ›› 2008, Vol. 29 ›› Issue (5): 416-419.

灵芝肽对小鼠半乳糖胺致肝损伤的保护作用

石燕玲, 何慧, 梁润生, 张胜, 郭辉, 黄迪惠

华中农业大学食品科技学院；华中农业大学食品科技学院

出版日期:2008-05-15 发布日期:2011-08-26

Hepatoprotective Effects of Ganoderma lucidum Peptides Against D-galactosamine-induced Liver Injury in Mice

SHI Yan-Ling, HE Hui, LIANG Run-Sheng, ZHANG Sheng, GUO Hui, HUANG Di-Hui

College of Food Science and Technology,Huazhong Agricultural University

Online:2008-05-15 Published:2011-08-26

摘要/Abstract

摘要： 目的:研究灵芝肽(GLP)对D-半乳糖胺(D-Gal)诱导的小鼠化学性肝损伤的保护作用。方法:将小鼠随机分成正常组、模型组、GLP低、中、高剂量组,每天灌胃一次,GLP组分别灌胃GLP60、120、180mg/kgbw,正常组、模型组灌胃等量生理盐水,2周后模型组和GLP组腹腔内注射D-Gal(750mg/kgbw),诱导小鼠肝损伤。通过试剂盒的方法分别检测各组小鼠血清中谷丙转氨酶(ALT)、谷草转氨酶(AST)活力,肝组织中丙二醛(MDA)、谷胱甘肽(GSH)含量及超氧化物歧化酶(SOD)活力;并进行肝组织病理学镜检。结果:与模型组比,以180mg/kgbw给予GLP,可显著降低小鼠血清ALT、AST的活性和肝组织中MDA含量,提高肝组织中SOD的活力与GSH的含量(p<0.05～0.01),肝组织病理学镜检结果显示已接近正常组。结论:GLP对D-Gal诱导的小鼠肝损伤有很好的保护作用。

关键词: 灵芝肽, D-氨基半乳糖, 肝损伤, 保肝

Abstract: Objective:To research the hepatoprotective effects of Ganoderma lucidum peptides(GLP) against D-galactosamine(D-Gal)induced liver damage in mice.Method:Mice were randomly divided into five groups,namely normal,model and three GLP-treated groups.GLP-treated groups were daily given(ig) the doses at 60,120 and 180 mg GLP /kg bw respectively.Normal and model groups simultaneously received the same amount of physiological saline for 2 weeks.Then the mice from model group and GLP-treated groups were treated with 750 mg/kg bw D-Gal(ip).Aspartate transaminase/alanine transaminase(AST/ALT) activities in serum,malondialdehyde/glutathione(MDA/GSH) levels and superoxidedismutase(SOD) activity in liver were measured by the detection kits according to the manufacturer’s protocols.The liver histopathological characters were observed under light microscopy.Results:At the dose of 180mg/kg bw GLP,the ALT/AST activities in serum and MDA lever in liver were significantly reduced,SOD activity and GSH content in liver were significantly increased(p<0.05～0.01) when compared to model group.The liver histopathological characters were close to normal groups’.Conclusion:GLP possesses potent hepatoprotective effects against D-Gal-induced liver damage in mice.

Key words: Ganoderma lucidum peptides(GLP), liver damage, D-Galactosamine(D-Gal), hepatoprotective effect

石燕玲, 何慧, 梁润生, 张胜, 郭辉, 黄迪惠. 灵芝肽对小鼠半乳糖胺致肝损伤的保护作用[J]. 食品科学, 2008, 29(5): 416-419.

SHI Yan-Ling, HE Hui, LIANG Run-Sheng, ZHANG Sheng, GUO Hui, HUANG Di-Hui. Hepatoprotective Effects of Ganoderma lucidum Peptides Against D-galactosamine-induced Liver Injury in Mice[J]. FOOD SCIENCE, 2008, 29(5): 416-419.

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