食品科学 ›› 2005, Vol. 26 ›› Issue (9): 116-119.

• 基础研究 • 上一篇    下一篇

培养条件对冰核生产菌Xanthomonas ampelina TS206产胶率的影响

 李鸿雁, 陈庆森, 阎亚丽, 刘磊, 崔维   

  1. 天津市食品生物技术重点实验室,天津商学院生物工程系
  • 出版日期:2005-09-15 发布日期:2011-09-20

Effect of Cultivation Condition on Xanthan Gum Ratio from Ice-nucleation Active Bacteria of Xanthomonas ampelina TS206

 LI  Hong-Yan, CHEN  Qing-Sen, YAN  Ya-Li, LIU  Lei, CUI  Wei   

  1. Tianjin Key Laboratory of Food Biotechnolgy, Department of Biotechnology, Tianjin University of Commerce
  • Online:2005-09-15 Published:2011-09-20

摘要: 培养条件影响冰核细菌的生物量、冰核活性和产胶率。通过单因子试验确定了TS206的最适培养条件:最适装液量为100ml,培养基的最适起始pH值为7.0,最佳培养时间为48h,但是通过上述培养条件的调整并不能明显降低黄原胶的产率。采取从30℃到18℃的双温培养可以在保持较高的生物量和冰核活性的前提下,降低黄原胶的产量,并明显缩短了培养时间。

关键词: 冰核活性细菌, 黄原胶, 培养条件, 双温培养

Abstract: The cultivation condition affected the cell growth, the ice nucleation activity and the xanthan gum ratio. Through single factor test ,the best culture condition of Xanthomonas ampelina TS206 was determined. The optimum initial pH value was 7.0, the optimum broth capacity was 100ml, the optimum cultivation time was 48h .But the xanthan gum ratio could not be reduced effectively by the variation of the above mentioned culture condition. During the cultivation, changing temperature from 30℃ to 18℃ not only promoted the biomass and the ice nucleation activity but also inhibited the xanthan gum ratio and reduced the culture time obviously.

Key words: ice-nucleation active bacteria, xanthan gum, cultivation condition, two segment temperature culture