双水相亲和萃取法从豆壳中分离过氧化物酶

食品科学 ›› 2004, Vol. 25 ›› Issue (7): 93-96.

双水相亲和萃取法从豆壳中分离过氧化物酶

文禹撷, 邹少兰, 林东强, 姚善泾

浙江大学化学工程与生物工程系，江西萍乡高等专科学校

出版日期:2004-07-15 发布日期:2011-10-24

Affinity Partition of Soybean Hull Peroxidase in Aqueous Two-phase Systems

WEN Yu-Xie, ZOU Shao-Lan, LIN Dong-Qiang, YAO Shan-Jing

1.Department of Chemical and Biochemical Engineering,Zhejiang University;2.Pingxiang College for Professional Training

Online:2004-07-15 Published:2011-10-24

摘要/Abstract

摘要： 采用双水相金属螯合亲和萃取法从豆壳中分离过氧化物酶,考察了豆壳过氧化物酶在不同类型双水相系统中金属螯合亲和配基聚乙二醇(PEG)-亚氨基二乙酸(IDA)-Cu(Ⅱ)对亲和分配的影响,发现PEG/羟丙基淀粉(PES)系统适合于金属螯合亲和分配豆壳过氧化物酶。进一步考察亲和配基加入量、成相聚合物分子量、系统组成、pH等因素对PEG/PES系统亲和分配的影响,优化分离条件为:PEG20009%,PES20014%,PEG-IDA-Cu(Ⅱ)1%,豆壳过氧化物酶的分配系数达40,纯化因子为2.8,回收率达到93%。

关键词: 亲和分配, 双水相系统, 金属螯合, 豆壳过氧化物酶

Abstract: Metal chelating affinity partition with aqueous two-phase system was studied to separate the peroxidase fromsoybean hull extract. The effects of affinity ligand (polyethylene glycol (PEG)- iminodiacetic acid (IDA)-Cu(II)) on the affinitypartition of soybean hull peroxidase (SHP) were investigated in different types of aqueous two-phase systems. The resultsindicated that PEG/ hydroxypropyl starch (PES) aqueous two-phase system was suitable for affinity extraction of SHP. Severalfactors, such as adding amount of affinity ligand, molecular weight of phase-forming polymer, system compositions, pH, etc.,were optimized. The best separation conditions were: PEG2000 9%, PES200 14% and PED-IDA-Cu(II) 1%. The partitioncoefficient of SHP could reach 40, and purification factor was 2.8 with the recovery of 93%.

Key words: affinity partition, aqueous two-phase system, metal chelating, soybean hull peroxidase

文禹撷, 邹少兰, 林东强, 姚善泾. 双水相亲和萃取法从豆壳中分离过氧化物酶[J]. 食品科学, 2004, 25(7): 93-96.

WEN Yu-Xie, ZOU Shao-Lan, LIN Dong-Qiang, YAO Shan-Jing. Affinity Partition of Soybean Hull Peroxidase in Aqueous Two-phase Systems[J]. FOOD SCIENCE, 2004, 25(7): 93-96.

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