食品科学 ›› 2006, Vol. 27 ›› Issue (12): 389-392.

• 工艺技术 • 上一篇    下一篇

乳酸菌富集硒的条件研究

刘文群,邓泽元,李静   

  1. 南昌大学食品科学教育部重点实验室;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Study on Conditions of Bioconcentrating Trace Element Se by Lactobacillus bulgaricus

LIU Wen-qun,DENG Ze-yuan,LI Jing   

  1. Key Laboratory of Food Science, Ministry of Education, Nanchang University, Nanchang 330047, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 研究了保加利亚乳杆菌将无机态微量元素硒富集转化为细胞内的有机态微量元素硒的条件。正交试验结果表明:培养基中亚硒酸钠浓度为主要影响因素,培养温度、培养时间和接种量为次要影响因素。在3个次要因素中,培养温度的影响是较主要的。最佳富集条件为亚硒酸钠浓度90μg/ml、培养温度37℃、培养时间60h、接种量8%。细胞富集的有机态硒占总富集量的80%以上。

关键词: 保加利亚乳杆菌, 微量元素硒, 富集条件

Abstract: In this paper, Lactobacillus bulgaricus (LB) was cultured in the medium with Se. The inorganic state trace element Se was changed into the organic state trace element through bio-transformation. The effect of different conditions on enrichment of trace element Se by LB was assessed with the orthogonal design of L9(34). The results showed that Na2SeO3 concentration in the medium was the main influence factor; the culture temperature, the culture time and the inoculation volume were the secondary influence factors. The optimal enriching conditions of Se by LB were as follows: Na2SeO3 concentration, 90μg/ml; the culture temperature, 37℃; the culture time, 60h; and the inoculation volume, 8%. The amount of organic form of Se was above 80%.

Key words: Lactobacillus bulgaricus, trace element Se, enriching conditions