食品科学 ›› 2006, Vol. 27 ›› Issue (12): 512-517.

• 工艺技术 • 上一篇    下一篇

培养条件及营养元素对开菲尔粒增殖的影响

任雅丽,李博,籍保平,季红,刘佳   

  1. 中国农业大学食品科学与营养工程学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Effect of the Different Incubations and the Nutrimental Resource on Kefir Grains Increase

 REN  Ya-Li, LI  Bo, JI  Bao-Ping, JI  Hong, LIU  Jia   

  1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 本文研究了清洗、搅拌等培养条件以及碳源、氮源和无机盐等营养元素对开菲尔粒增殖的影响。实验结果表明,当开菲尔粒的接种量为5%、培养温度为25℃时,对开菲尔粒清洗会降低其增殖率;搅拌培养可以显著提高增值率:与对照组相比,可使增殖率提高2倍。外加碳源不利于开菲尔粒生长;添加0.002%L-蛋氨酸、0.5%胰蛋白胨和0.5%酵母浸提物对开菲尔粒增殖作用显著;无机盐的添加也有利于增值率的提高。当添加0.001%MnSO4·H2O、0.002%MgSO4·7H2O和0.001%K2HPO4·3H2O时,可比对照组的增值率提高0.5倍。增殖后的开菲尔粒的发酵特性及菌群比例均没有变化。

关键词: 开菲尔粒, 增殖, 搅拌, 清洗, 营养素

Abstract: In this paper, the effect of kefir grains increase with different incubations and nutrimental resource was studied. The result showed that, when inoculated ratio of kefir grains was 5%, incubation temperature of 25℃, multiplication was lowed with rinsing or kefir grains preserved at 4℃, doubled mass grains produced with agitation. The grains increase was little with single carbon resource, even restrained with compound carbon resource in a sense. Factors influencing grains increase — enrichment with L-methionine 0.002%, tryptose 0.5% and yeast extract 0.5%, and with MnSO·4H2O 0.001%, MgSO·47H2O 0.002% and K2HPO·43H2O 0.001%. Fermented characteristic and flora proportion of kefir grains were no changes after increasing.

Key words: kefir grains, increase, agitation, rinse, nutrient addition