食品科学 ›› 2006, Vol. 27 ›› Issue (12): 572-575.

• 工艺技术 • 上一篇    下一篇

香菇富锌及其培养条件优化

叶明,陈辉,章建国,储棂椤   

  1. 合肥工业大学生物与食品工程学院;
  • 出版日期:2006-12-15 发布日期:2011-11-23

Study on Optimization of Cultivation Condition in Zinc-enriched Lentinula edodes

 YE  Ming, CHEN  Hui, ZHANG  Jian-Guo, CHU  Ling-Luo   

  1. College of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China
  • Online:2006-12-15 Published:2011-11-23

摘要: 对香菇双单杂交菌株QS29、NN22、QQ06、QQ05进行了富锌培养,确定了其生长耐受锌的浓度范围为0.05~0.3mg/ml。选择香菇菌株NN22进行了培养条件优化与液体出菇试验,结果表明:香菇菌株NN22在PDY培养基中富锌的适宜培养条件为0.1mg/mlZn2+,25℃,pH7.0;在此条件下,1g菌丝(干重)的锌含量达到9.26mg,多糖含量为5.19mg;富锌香菇菌株NN22在0.1mg/mlZn2+PDY培养基里第19d开始出现菇蕾,较未富锌菌株出菇时间缩短了6d。

关键词:  , 香菇, 富锌, 培养条件优化, 出菇试验

Abstract: Four strains(QS29, NN22, QQ06, QQ05) of di-mon mating of Lentinula edodes were Zinc-enriched in the medium with Zn2+ content ranged from 0.05 to 0.3mg/ml. The optimization of cultivation conditions of the strain NN22 was selected, and primodium test of the Zinc-enriched strain NN22 was carried out in Zn2+ of PDY. The results showed that the highest Zn2+ content of the strain NN22 was obtained within 20 days at 25℃ and initial pH7.0 in 0.1mg/ml Zn2+ of PDY. Under the optimized cultivation conditions, Zn2+ content and polysaccharide content of the strain NN22 reached 9.26mg/g and 5.19mg/g, respectively. The phase of primodium formed of the Zinc-enriched strain NN22 was 19 days in 0.1mg/ml Zn2+ of PDY, which was reduced 6 days compared to the original strain.

Key words: Lentinula edodes, zinc-enriched, optimization of cultivation condition, primodium test